PMID- 10917878
OWN - NLM
STAT- MEDLINE
DCOM- 20000817
LR  - 20171116
IS  - 1684-1182 (Print)
IS  - 1684-1182 (Linking)
VI  - 33
IP  - 2
DP  - 2000 Jun
TI  - Soluble CD4 suppresses delayed-type hypersensitivity reaction of CD4 loosing mice 
      by inhibiting INFgamma production.
PG  - 93-9
AB  - The CD4 loosing (CD4L) mice have a novel defect in CD4 expression where CD4 mRNA 
      is alternatively spliced to delete a transmembrane portion to give rise to the 
      secretion of soluble CD4 (sCD4) without the expression of membrane-bound CD4. To 
      analyze the role of sCD4 in immune responses in CD4L mice, experiments have been 
      done by comparing CD4L mice with CD4 knockout (CD4KO) mice on the same C57BL/6 
      background. Significantly reduced delayed-type hypersensitivity (DTH) response 
      against methylated BSA (mBSA) was found in CD4L mice; however, CD4KO mice showed 
      a comparable response to wild type mice. Anti-CD4 treatment in CD4L mice could 
      restore DTH response. Moreover, implantation of CD4KO mice with a diffusion 
      chamber containing sCD4 secreting cells suppressed DTH response. DTH response 
      recovered by anti-CD4 treatment in CD4L mice was inhibited by anti-interferon 
      gamma (IFNgamma). IFNgamma production of mBSA-stimulated lymph node cells was 
      significantly reduced in CD4L mice as compared with that in CD4KO or wild type 
      mice, and the reduction could be recovered by the anti-CD4 treatment. IFNgamma 
      production of mBSA-stimulated lymph node cells was suppressed in CD4KO mice 
      carrying a diffusion chamber containing sCD4 secreting cells as compared with 
      those containing control cells. Taken together, results in this study strongly 
      indicate that sCD4 suppresses DTH responses of CD4L mice by inhibiting IFNgamma 
      production. The mutant mice could provide a good model to analyze the mechanism 
      of IFNgamma involvement in the DTH response.
FAU - Wang, C R
AU  - Wang CR
AD  - Department of Internal Medicine, National Cheng Kung University Hospital, Tainan, 
      Taiwan, ROC.
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Microbiol Immunol Infect
JT  - Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi
JID - 100956211
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (CD4 Antigens)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/immunology
MH  - CD4 Antigens/*physiology
MH  - Hypersensitivity, Delayed/*prevention & control
MH  - Interferon-gamma/*biosynthesis
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
EDAT- 2000/08/05 11:00
MHDA- 2000/08/19 11:00
CRDT- 2000/08/05 11:00
PHST- 2000/08/05 11:00 [pubmed]
PHST- 2000/08/19 11:00 [medline]
PHST- 2000/08/05 11:00 [entrez]
PST - ppublish
SO  - J Microbiol Immunol Infect. 2000 Jun;33(2):93-9.