PMID- 10918389
OWN - NLM
STAT- MEDLINE
DCOM- 20000920
LR  - 20220409
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 29
IP  - 1
DP  - 2000 Sep
TI  - Mapping of the chromosome 19 q-arm glioma tumor suppressor gene using 
      fluorescence in situ hybridization and novel microsatellite markers.
PG  - 16-25
AB  - Allelic loss of chromosome arm 19q is a frequent event in human diffuse glioma, 
      suggesting the presence of a tumor suppressor gene. Previous loss of 
      heterozygosity (LOH) analyses have mapped this gene to a 1.4-megabase interval, 
      between the genetic markers D19S412 and STD. Further narrowing of this interval 
      has been limited by the resolution of mapped polymorphic markers. In the present 
      study, we have used genomic clones mapped to 19q as fluorescence in situ 
      hybridization (FISH) probes to map the breakpoints of 13 gliomas with 19q13.3 
      deletion boundaries. In addition, we have developed three new polymorphic 
      microsatellite markers (D19S1180, D19S1181, and D19S1182) that map between 
      D19S412 and STD and have used these new markers to identify two gliomas with 
      small deletions between the D19S412 and STD markers. Collectively, these data 
      suggest that the region of common deletion may be as narrow as 150 kb and should 
      facilitate future efforts to identify the glioma 19q tumor suppressor gene.
CI  - Copyright 2000 Wiley-Liss, Inc.
FAU - Smith, J S
AU  - Smith JS
AD  - Department of Laboratory Medicine and Pathology, Division of Laboratory Genetics, 
      Mayo Clinic and Foundation, Rochester, Minnesota 55905, USA.
FAU - Tachibana, I
AU  - Tachibana I
FAU - Lee, H K
AU  - Lee HK
FAU - Qian, J
AU  - Qian J
FAU - Pohl, U
AU  - Pohl U
FAU - Mohrenweiser, H W
AU  - Mohrenweiser HW
FAU - Borell, T J
AU  - Borell TJ
FAU - Hosek, S M
AU  - Hosek SM
FAU - Soderberg, C L
AU  - Soderberg CL
FAU - von Deimling, A
AU  - von Deimling A
FAU - Perry, A
AU  - Perry A
FAU - Scheithauer, B W
AU  - Scheithauer BW
FAU - Louis, D N
AU  - Louis DN
FAU - Jenkins, R B
AU  - Jenkins RB
LA  - eng
GR  - CA50905/CA/NCI NIH HHS/United States
GR  - CA57683/CA/NCI NIH HHS/United States
GR  - CA69285/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
SB  - IM
MH  - Chromosome Deletion
MH  - Chromosome Mapping/methods
MH  - Chromosomes, Human, Pair 19/*genetics
MH  - Cloning, Molecular
MH  - Female
MH  - Genes, Tumor Suppressor/*genetics
MH  - Glioma/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Loss of Heterozygosity
MH  - Male
MH  - Microsatellite Repeats/*genetics
EDAT- 2000/08/05 11:00
MHDA- 2000/09/23 11:01
CRDT- 2000/08/05 11:00
PHST- 2000/08/05 11:00 [pubmed]
PHST- 2000/09/23 11:01 [medline]
PHST- 2000/08/05 11:00 [entrez]
AID - 10.1002/1098-2264(2000)9999:9999<::AID-GCC1007>3.0.CO;2-I [pii]
AID - 10.1002/1098-2264(2000)9999:9999<::aid-gcc1007>3.3.co;2-9 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 2000 Sep;29(1):16-25. doi: 
      10.1002/1098-2264(2000)9999:9999<::aid-gcc1007>3.3.co;2-9.