PMID- 10918392
OWN - NLM
STAT- MEDLINE
DCOM- 20000920
LR  - 20191025
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 29
IP  - 1
DP  - 2000 Sep
TI  - MLL amplification in myeloid leukemias: A study of 14 cases with multiple copies 
      of 11q23.
PG  - 40-7
AB  - We here report the clinical, cytogenetic, fluorescence in situ hybridization 
      (FISH), and Southern blot data on 14 patients with a myeloid malignancy and 
      structural aberration of chromosome band 11q23 associated with overrepresentation 
      or amplification of the MLL gene. The number of copies of MLL varied from three 
      (two cases) to a cluster consisting of multiple hybridization spots. Together 
      with previous reports, available data indicate that amplification of 11q23/MLL is 
      a recurrent genetic change in myeloid malignancy. It affects mainly elderly 
      patients and is often associated with dysplastic bone marrow changes or with 
      complex karyotypic aberrations, suggestive of genotoxic exposure. It is 
      associated with a poor prognosis. In addition, FISH analysis of nine cases with 
      additional 11q probes showed that the overrepresented chromosomal region is 
      generally not restricted to MLL, and Southern blot analysis indicated that 
      amplification does not involve a rearranged copy of this gene. The significance 
      of MLL amplification and the mechanisms by which it could play a role in 
      leukemogenesis and/or disease progression remain to be elucidated.
CI  - Copyright 2000 Wiley-Liss, Inc.
FAU - Michaux, L
AU  - Michaux L
AD  - Center for Human Genetics, University of Leuven, Leuven, Belgium.
FAU - Wlodarska, I
AU  - Wlodarska I
FAU - Stul, M
AU  - Stul M
FAU - Dierlamm, J
AU  - Dierlamm J
FAU - Mugneret, F
AU  - Mugneret F
FAU - Herens, C
AU  - Herens C
FAU - Beverloo, B
AU  - Beverloo B
FAU - Verhest, A
AU  - Verhest A
FAU - Verellen-Dumoulin, C
AU  - Verellen-Dumoulin C
FAU - Verhoef, G
AU  - Verhoef G
FAU - Selleslag, D
AU  - Selleslag D
FAU - Madoe, V
AU  - Madoe V
FAU - Lecomte, M
AU  - Lecomte M
FAU - Deprijck, B
AU  - Deprijck B
FAU - Ferrant, A
AU  - Ferrant A
FAU - Delannoy, A
AU  - Delannoy A
FAU - Marichal, S
AU  - Marichal S
FAU - Duhem, C
AU  - Duhem C
FAU - Dicato, M
AU  - Dicato M
FAU - Hagemeijer, A
AU  - Hagemeijer A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (KMT2A protein, human)
RN  - 0 (Transcription Factors)
RN  - 149025-06-9 (Myeloid-Lymphoid Leukemia Protein)
RN  - EC 2.1.1.43 (Histone-Lysine N-Methyltransferase)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Blotting, Southern
MH  - Chromosomes, Human, Pair 11/*genetics
MH  - DNA-Binding Proteins/*genetics
MH  - Female
MH  - Gene Amplification/*genetics
MH  - Gene Dosage
MH  - Histone-Lysine N-Methyltransferase
MH  - Humans
MH  - Immunophenotyping
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Leukemia, Myeloid/diagnosis/*genetics/*pathology
MH  - Male
MH  - Middle Aged
MH  - Myeloid-Lymphoid Leukemia Protein
MH  - *Proto-Oncogenes
MH  - Retrospective Studies
MH  - *Transcription Factors
EDAT- 2000/08/05 11:00
MHDA- 2000/09/23 11:01
CRDT- 2000/08/05 11:00
PHST- 2000/08/05 11:00 [pubmed]
PHST- 2000/09/23 11:01 [medline]
PHST- 2000/08/05 11:00 [entrez]
AID - 10.1002/1098-2264(2000)9999:9999<::AID-GCC1003>3.0.CO;2-U [pii]
AID - 10.1002/1098-2264(2000)9999:9999<::aid-gcc1003>3.3.co;2-l [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 2000 Sep;29(1):40-7. doi: 
      10.1002/1098-2264(2000)9999:9999<::aid-gcc1003>3.3.co;2-l.