PMID- 10919687
OWN - NLM
STAT- MEDLINE
DCOM- 20001205
LR  - 20220331
IS  - 0142-9612 (Print)
IS  - 0142-9612 (Linking)
VI  - 21
IP  - 18
DP  - 2000 Sep
TI  - In vitro and in vivo degradation of porous poly(DL-lactic-co-glycolic acid) 
      foams.
PG  - 1837-45
AB  - This study investigated the in vitro degradation of porous 
      poly(DL-lactic-co-glycolic acid) (PLGA) foams during a 20-week period in pH 7.4 
      phosphate-buffered saline (PBS) at 37 degrees C and their in vivo degradation 
      following implantation in rat mesentery for up to 8 weeks. Three types of PLGA 85 
      : 15 and three types of 50 : 50 foams were fabricated using a solvent-casting, 
      particulate-leaching technique. The two types had initial salt weight fraction of 
      80 and 90%, and a salt particle size of 106-150 microm, while the third type had 
      90% initial weight fraction of salt in the size range 0-53 microm. The porosities 
      of the resulting foams were 0.82, 0.89, and 0.85 for PLGA 85 : 15, and 0.73, 
      0.87, and 0.84 for PLGA 50 : 50 foams, respectively. The corresponding median 
      pore diameters were 30, 50, and 17 microm for PLGA 85: 15, and 19, 17, and 17 
      microm for PLGA 50 : 50. The in vitro and in vivo degradation kinetics of PLGA 
      85: 15 foams were independent of pore morphology with insignificant variation in 
      foam weight, thickness, pore distribution, compressive creep behavior, and 
      morphology during degradation. The in vitro foam half-lives based on the weight 
      average molecular weight were 11.1 +/- 1.8 (80%, 106-150 microm), 12.0 +/- 2.0 
      (90%, 106-150 microm), and 11.6 +/- 1.3 (90%, 0-53 microm) weeks, similar to the 
      corresponding values of 9.4 +/- 2.2, 14.3 +/- 1.5, and 13.7 +/- 3.3 weeks for in 
      vivo degradation. In contrast, all PLGA 50 : 50 foams exhibited significant 
      change in foam weight, water absorption, and pore distribution after 6-8 weeks of 
      incubation with PBS. The in vitro foam half-lives were 3.3 +/- 0.3 (80%, 106-150 
      microm), 3.0 +/- 0.3 (90%, 106-150 microm), and 3.2 +/- 0.1 (90%, 0-53 microm) 
      weeks, and the corresponding in vivo half-lives were 1.9 micro 0.1, 2.2 +/- 0.2, 
      and 2.4 +/- 0.2 weeks. The significantly shorter half-lives of PLGA 50: 50 
      compared to 85: 15 foams indicated their faster degradation both in vitro and in 
      vivo. In addition, PLGA 50: 50 foams exhibited significantly faster degradation 
      in vivo as compared to in vitro conditions due to an autocatalytic effect of the 
      accumulated acidic degradation products in the medium surrounding the implants. 
      These results suggest that the polymer composition and environmental conditions 
      have significant effects on the degradation rate of porous PLGA foams.
FAU - Lu, L
AU  - Lu L
AD  - Department of Bioengineering, Rice University, Houston, TX 77005-1892, USA.
FAU - Peter, S J
AU  - Peter SJ
FAU - Lyman, M D
AU  - Lyman MD
FAU - Lai, H L
AU  - Lai HL
FAU - Leite, S M
AU  - Leite SM
FAU - Tamada, J A
AU  - Tamada JA
FAU - Uyama, S
AU  - Uyama S
FAU - Vacanti, J P
AU  - Vacanti JP
FAU - Langer, R
AU  - Langer R
FAU - Mikos, A G
AU  - Mikos AG
LA  - eng
GR  - R01-AR44381/AR/NIAMS NIH HHS/United States
GR  - R01-DE13031/DE/NIDCR NIH HHS/United States
GR  - R29-AR42639/AR/NIAMS NIH HHS/United States
GR  - etc.
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (Biocompatible Materials)
RN  - 0 (Polymers)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - FXS1BY2PGL (Mercury)
SB  - IM
MH  - Animals
MH  - Biocompatible Materials/*chemistry/*pharmacokinetics
MH  - Biodegradation, Environmental
MH  - Half-Life
MH  - Hot Temperature
MH  - Hydrogen-Ion Concentration
MH  - Kinetics
MH  - Lactic Acid/*chemistry/*pharmacokinetics
MH  - Mercury
MH  - Mesentery/cytology/pathology
MH  - Polyglycolic Acid/*chemistry/*pharmacokinetics
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Polymers/*chemistry/*pharmacokinetics
MH  - Prostheses and Implants
MH  - Rats
MH  - Thermodynamics
EDAT- 2000/08/05 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/08/05 11:00
PHST- 2000/08/05 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/08/05 11:00 [entrez]
AID - S0142961200000478 [pii]
AID - 10.1016/s0142-9612(00)00047-8 [doi]
PST - ppublish
SO  - Biomaterials. 2000 Sep;21(18):1837-45. doi: 10.1016/s0142-9612(00)00047-8.