PMID- 10919787
OWN - NLM
STAT- MEDLINE
DCOM- 20010104
LR  - 20210526
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 66
IP  - 8
DP  - 2000 Aug
TI  - Genotypic heterogeneity of Streptococcus oralis and distinct aciduric 
      subpopulations in human dental plaque.
PG  - 3330-6
AB  - The genotypic heterogeneity of Streptococcus oralis isolated from the oral cavity 
      was investigated using repetitive extragenic palindromic PCR. Unrelated subjects 
      harbored unique genotypes, with numerous genotypes being isolated from an 
      individual. S. oralis is the predominant aciduric bacterium isolated from 
      noncarious tooth sites. Genotypic comparison of the aciduric populations isolated 
      at pH 5.2 with those isolated from mitis-salivarius agar (MSA) (pH 7.0) indicated 
      that the aciduric populations were genotypically distinct in the majority of 
      subjects (chi(2) = 13.09; P = 0.0031). Neither the aciduric nor the MSA-isolated 
      strains were stable, with no strains isolated at baseline being isolated 4 or 12 
      weeks later in the majority of subjects. The basis of this instability is unknown 
      but is similar to that reported for Streptococcus mitis. Examination of S. oralis 
      strains isolated from cohabiting couples demonstrated that in three of five 
      couples, genotypically identical strains were isolated from both partners and 
      this was confirmed by using Salmonella enteritidis repetitive element PCR and 
      enterobacterial PCR typing. These data provide further evidence of the 
      physiological and genotypic heterogeneity of non-mutans streptococci. The 
      demonstration of distinct aciduric populations of S. oralis implies that the role 
      of these and other non-mutans streptococci in the caries process requires 
      reevaluation.
FAU - Alam, S
AU  - Alam S
AD  - Dental Caries Research Group, Guy's, King's, and St. Thomas' Dental Institute, 
      London, England.
FAU - Brailsford, S R
AU  - Brailsford SR
FAU - Adams, S
AU  - Adams S
FAU - Allison, C
AU  - Allison C
FAU - Sheehy, E
AU  - Sheehy E
FAU - Zoitopoulos, L
AU  - Zoitopoulos L
FAU - Kidd, E A
AU  - Kidd EA
FAU - Beighton, D
AU  - Beighton D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
SB  - IM
MH  - Dental Plaque/*microbiology
MH  - Female
MH  - *Genetic Variation
MH  - Genotype
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Male
MH  - Polymerase Chain Reaction/methods
MH  - Saliva/microbiology
MH  - Streptococcal Infections/*microbiology/transmission
MH  - Streptococcus oralis/*classification/genetics/*isolation & purification
PMC - PMC92151
EDAT- 2000/08/05 11:00
MHDA- 2001/02/28 10:01
PMCR- 2000/08/01
CRDT- 2000/08/05 11:00
PHST- 2000/08/05 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/08/05 11:00 [entrez]
PHST- 2000/08/01 00:00 [pmc-release]
AID - 0589 [pii]
AID - 10.1128/AEM.66.8.3330-3336.2000 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2000 Aug;66(8):3330-6. doi: 
      10.1128/AEM.66.8.3330-3336.2000.