PMID- 10919825
OWN - NLM
STAT- MEDLINE
DCOM- 20010104
LR  - 20210526
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 66
IP  - 8
DP  - 2000 Aug
TI  - Community structure, cellular rRNA content, and activity of sulfate-reducing 
      bacteria in marine arctic sediments.
PG  - 3592-602
AB  - The community structure of sulfate-reducing bacteria (SRB) of a marine Arctic 
      sediment (Smeerenburgfjorden, Svalbard) was characterized by both fluorescence in 
      situ hybridization (FISH) and rRNA slot blot hybridization by using group- and 
      genus-specific 16S rRNA-targeted oligonucleotide probes. The SRB community was 
      dominated by members of the Desulfosarcina-Desulfococcus group. This group 
      accounted for up to 73% of the SRB detected and up to 70% of the SRB rRNA 
      detected. The predominance was shown to be a common feature for different 
      stations along the coast of Svalbard. In a top-to-bottom approach we aimed to 
      further resolve the composition of this large group of SRB by using probes for 
      cultivated genera. While this approach failed, directed cloning of probe-targeted 
      genes encoding 16S rRNA was successful and resulted in sequences which were all 
      affiliated with the Desulfosarcina-Desulfococcus group. A group of clone 
      sequences (group SVAL1) most closely related to Desulfosarcina variabilis (91.2% 
      sequence similarity) was dominant and was shown to be most abundant in situ, 
      accounting for up to 54. 8% of the total SRB detected. A comparison of the two 
      methods used for quantification showed that FISH and rRNA slot blot hybridization 
      gave comparable results. Furthermore, a combination of the two methods allowed us 
      to calculate specific cellular rRNA contents with respect to localization in the 
      sediment profile. The rRNA contents of Desulfosarcina-Desulfococcus cells were 
      highest in the first 5 mm of the sediment (0.9 and 1.4 fg, respectively) and 
      decreased steeply with depth, indicating that maximal metabolic activity occurred 
      close to the surface. Based on SRB cell numbers, cellular sulfate reduction rates 
      were calculated. The rates were highest in the surface layer (0.14 fmol cell(-1) 
      day(-1)), decreased by a factor of 3 within the first 2 cm, and were relatively 
      constant in deeper layers.
FAU - Ravenschlag, K
AU  - Ravenschlag K
AD  - Molecular Ecology Group, Max Planck Institute for Marine Microbiology, 28359 
      Bremen, Germany.
FAU - Sahm, K
AU  - Sahm K
FAU - Knoblauch, C
AU  - Knoblauch C
FAU - Jorgensen, B B
AU  - Jorgensen BB
FAU - Amann, R
AU  - Amann R
LA  - eng
SI  - GENBANK/AF233491
SI  - GENBANK/AF233492
SI  - GENBANK/AF233493
SI  - GENBANK/AF233494
SI  - GENBANK/AF233495
SI  - GENBANK/AF233496
SI  - GENBANK/AF233497
SI  - GENBANK/AF233498
SI  - GENBANK/AF233499
SI  - GENBANK/AF233500
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sulfates)
SB  - IM
MH  - Arctic Regions
MH  - DNA, Bacterial/analysis/genetics
MH  - DNA, Ribosomal/analysis/genetics
MH  - *Ecosystem
MH  - Fermentation
MH  - Genes, rRNA
MH  - Geologic Sediments/*microbiology
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - Nucleic Acid Hybridization
MH  - Oxidation-Reduction
MH  - Phylogeny
MH  - Polymerase Chain Reaction
MH  - RNA, Bacterial/analysis
MH  - RNA, Ribosomal, 16S/*analysis/genetics
MH  - Sulfates/*metabolism
MH  - Sulfur-Reducing Bacteria/chemistry/genetics/*isolation & purification/metabolism
PMC - PMC92189
EDAT- 2000/08/05 11:00
MHDA- 2001/02/28 10:01
PMCR- 2000/08/01
CRDT- 2000/08/05 11:00
PHST- 2000/08/05 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/08/05 11:00 [entrez]
PHST- 2000/08/01 00:00 [pmc-release]
AID - 0372 [pii]
AID - 10.1128/AEM.66.8.3592-3602.2000 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2000 Aug;66(8):3592-602. doi: 
      10.1128/AEM.66.8.3592-3602.2000.