PMID- 10921044
OWN - NLM
STAT- MEDLINE
DCOM- 20000811
LR  - 20061115
IS  - 0253-3766 (Print)
IS  - 0253-3766 (Linking)
VI  - 20
IP  - 1
DP  - 1998 Jan
TI  - [Establishment of two human esophageal carcinoma cell lines and their cytogenetic 
      analysis].
PG  - 5-8
AB  - OBJECTIVE: To explore the relation between chromosomal aberration and 
      carcinogenesis of the esophagus by molecular cytogenetic analysis of two 
      esophageal carcinoma cell lines. METHODS: Small tissue blocks taken from resected 
      specimens of patients with esophageal cancer (EC) were cultured in medium 199 
      added with 1.5% fetal bovine serun, and two esophageal carcinoma cell lines, 
      EC8712 and EC8733, were established in 1987. They grew in monolayers. Molecular 
      cytogenetic studies were performed on the two cell lines using chromosome 
      G-banding, fluorescence in situ hybridization (FISH) and comparative gene 
      hybridization (CGH) techniques. RESULTS: Both cell lines were aneuploid. Loss of 
      chromosome Y, partial deletion of 1p, translocation of 2q and amplification of 
      5p, were observed in the 2 cell lines. Amplification of 8q and 13q in EC8733 and 
      deletion of 17p in EC8712 were detected. CONCLUSION: The presene of the reported 
      chromosomal aberrations may be related to the carcinogenesis and cancer 
      development of the esophagus.
FAU - Wang, X
AU  - Wang X
AD  - Department of Cell Biology, Chinese Academy of Medical Sciences, Peking Union 
      Medical College, Beijing.
FAU - Xiao, F
AU  - Xiao F
FAU - Wang, M
AU  - Wang M
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Zhong Liu Za Zhi
JT  - Zhonghua zhong liu za zhi [Chinese journal of oncology]
JID - 7910681
SB  - IM
MH  - Aneuploidy
MH  - Carcinoma, Squamous Cell/*genetics/pathology
MH  - *Chromosome Aberrations
MH  - Chromosome Deletion
MH  - Esophageal Neoplasms/*genetics/pathology
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Translocation, Genetic
MH  - Tumor Cells, Cultured
MH  - Y Chromosome
EDAT- 2000/08/02 11:00
MHDA- 2000/08/19 11:00
CRDT- 2000/08/02 11:00
PHST- 2000/08/02 11:00 [pubmed]
PHST- 2000/08/19 11:00 [medline]
PHST- 2000/08/02 11:00 [entrez]
PST - ppublish
SO  - Zhonghua Zhong Liu Za Zhi. 1998 Jan;20(1):5-8.