PMID- 10921098
OWN - NLM
STAT- MEDLINE
DCOM- 20000811
LR  - 20091119
IS  - 0253-2727 (Print)
IS  - 0253-2727 (Linking)
VI  - 19
IP  - 1
DP  - 1998 Jan
TI  - [Detection of CBF beta-MYH11 fusion transcript and inv(16)(p13;q22) in acute 
      myelomonocytic leukemia(M4) by RT-PCR and FISH].
PG  - 23-6
AB  - OBJECTIVE: To study the clinical significance of inv(16) and CBF beta-MYH11 
      fusion gene in the diagnosis and prognosis for M4Eo. METHODS: CBF beta-MYH11 
      fusion transcripts and inv (16) were analyzed by reverse-transcriptase polymerase 
      chain reaction(RT-PCR) and fluorescence in situ hybridization(FISH), 
      respectively, in acute myelomonocytic leukemia(M4) with or without eosinophilia. 
      RESULTS: In fifteen cases tested, one case of M4Eo and one of 9 M4 without 
      eosinophilia were found to have CBF beta-MYH11 fusion transcript. Follow-up of 
      the M4Eo patient showed residual PCR positivity 2 months after complete 
      remission. CONCLUSION: The data suggest that screening by both RT-PCR and FISH 
      should be performed in all AML-M4 regardless of morphologic features to allow 
      accurate diagnosis and prognosis of M4 patients.
FAU - He, K
AU  - He K
AD  - Shanghai Institute of Hematology, Shanghai Second Medical University.
FAU - Gu, B
AU  - Gu B
FAU - Cao, Q
AU  - Cao Q
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Xue Ye Xue Za Zhi
JT  - Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi
JID - 8212398
RN  - 0 (CBFbeta-MYH11 fusion protein)
RN  - 0 (Oncogene Proteins, Fusion)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Artificial Gene Fusion
MH  - Child
MH  - Child, Preschool
MH  - *Chromosome Inversion
MH  - *Chromosomes, Human, Pair 16
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia, Myelomonocytic, Acute/*genetics
MH  - Male
MH  - Middle Aged
MH  - Oncogene Proteins, Fusion/*genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Transcription, Genetic
EDAT- 2000/08/02 11:00
MHDA- 2000/08/19 11:00
CRDT- 2000/08/02 11:00
PHST- 2000/08/02 11:00 [pubmed]
PHST- 2000/08/19 11:00 [medline]
PHST- 2000/08/02 11:00 [entrez]
PST - ppublish
SO  - Zhonghua Xue Ye Xue Za Zhi. 1998 Jan;19(1):23-6.