PMID- 10921952
OWN - NLM
STAT- MEDLINE
DCOM- 20000920
LR  - 20200724
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 38
IP  - 8
DP  - 2000 Aug
TI  - Mapping of IS6110 insertion sites in two epidemic strains of Mycobacterium 
      tuberculosis.
PG  - 2923-8
AB  - A widely distributed strain designated 210 was identified in a study of the 
      diversity of Mycobacterium tuberculosis DNA fingerprints from three 
      geographically separate states in the United States. This strain is characterized 
      by a 21-band fingerprint pattern when probed with IS6110, and the pattern is 
      similar to that displayed by strains designated W. Intracellular growth of strain 
      210 isolates in human macrophages is significantly faster than that of isolates 
      from other clusters or nonclustered isolates. The purpose of this study was to 
      identify the sites of IS6110 insertions in strain 210 and compare these to IS6110 
      insertion sites in strain W. Our hypothesis is that an IS6110 insertion site(s) 
      could possibly be responsible for a strain's increased capacity for transmission 
      and/or replication. In this report, the insertion sites in strains 210 and W are 
      described and referenced to their location in the M. tuberculosis H37Rv genome 
      sequence. The W and 210 strains have 17 identical sites of IS6110 insertion and 
      additional sequence not found in H37Rv but present in other clinical isolates. 
      The IS6110 insertion site in the 36-bp direct repeat (DR) region of strains 210 
      and W has 15 spacers in the left flanking region. The DR region on the right side 
      of IS6110 has been deleted. Five sites of insertion in strain 210 not found in 
      strain W are described, as well as two unique sites in strain W. One copy of 
      IS6110 was found to reside 55 bp in the ctpD gene. This gene is expressed, 
      indicating that IS6110 can provide a promoter sequence for the transcription of 
      genes.
FAU - Beggs, M L
AU  - Beggs ML
AD  - Departments of Pathology, University of Arkansas for Medical Sciences and J. L. 
      McClellan Memorial Veterans Hospital, Little Rock, Arkansas 72205, USA. 
      BeggsMarjorieL@exchange.uams.edu
FAU - Eisenach, K D
AU  - Eisenach KD
FAU - Cave, M D
AU  - Cave MD
LA  - eng
SI  - GENBANK/AF227479
SI  - GENBANK/AF227480
SI  - GENBANK/AF227481
SI  - GENBANK/AF227482
SI  - GENBANK/AF227483
SI  - GENBANK/AF227484
SI  - GENBANK/AF227485
SI  - GENBANK/AF227486
SI  - GENBANK/AF227487
SI  - GENBANK/AF227488
SI  - GENBANK/AF227489
SI  - GENBANK/AF227490
SI  - GENBANK/AF227491
SI  - GENBANK/AF227492
SI  - GENBANK/AF227493
SI  - GENBANK/AF227494
SI  - GENBANK/AF227495
SI  - GENBANK/AF227496
SI  - GENBANK/AF227497
SI  - GENBANK/AF228671
SI  - GENBANK/AF228676
SI  - GENBANK/AF228677
SI  - GENBANK/AF228678
SI  - GENBANK/AF230202
SI  - GENBANK/AF230209
SI  - GENBANK/AF257113
SI  - GENBANK/AF257114
SI  - GENBANK/AF270486
PT  - Journal Article
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (DNA Transposable Elements)
SB  - IM
MH  - Base Sequence
MH  - Blotting, Southern
MH  - *Chromosome Mapping
MH  - DNA Fingerprinting
MH  - *DNA Transposable Elements
MH  - *Disease Outbreaks
MH  - Gene Expression
MH  - Humans
MH  - Molecular Sequence Data
MH  - Mycobacterium tuberculosis/classification/*genetics/isolation & purification
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tuberculosis, Pulmonary/epidemiology/*microbiology
PMC - PMC87149
EDAT- 2000/08/02 11:00
MHDA- 2000/09/23 11:01
PMCR- 2000/08/01
CRDT- 2000/08/02 11:00
PHST- 2000/08/02 11:00 [pubmed]
PHST- 2000/09/23 11:01 [medline]
PHST- 2000/08/02 11:00 [entrez]
PHST- 2000/08/01 00:00 [pmc-release]
AID - 0127 [pii]
AID - 10.1128/JCM.38.8.2923-2928.2000 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2000 Aug;38(8):2923-8. doi: 10.1128/JCM.38.8.2923-2928.2000.