PMID- 10921978
OWN - NLM
STAT- MEDLINE
DCOM- 20000920
LR  - 20200724
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 38
IP  - 8
DP  - 2000 Aug
TI  - Diagnostic utility of a multiplex herpesvirus PCR assay performed with 
      cerebrospinal fluid from human immunodeficiency virus-infected patients with 
      neurological disorders.
PG  - 3061-7
AB  - We used a multiplex nested-PCR assay for the simultaneous detection in 
      cerebrospinal fluid (CSF) of five human herpesviruses (HVs) (cytomegalovirus 
      [CMV], Epstein-Barr virus [EBV], varicella-zoster virus [VZV], herpes simplex 
      virus [HSV], and human herpesvirus 6 [HHV-6]) in a clinical evaluation of human 
      immunodeficiency virus (HIV)-infected patients with neurological disorders. This 
      method, which has the advantages of being rapid and economical, would be of 
      particular interest for the diagnosis of neurological syndromes caused by more 
      than one HV. We studied 251 CSF samples from 219 patients. HV DNA was 
      demonstrated in 93 (37%) of the CSF samples (34% of the patients). CMV was the HV 
      most frequently detected in our patients (25%), while EBV, VZV, HSV, and HHV-6 
      DNAs were present in significantly fewer cases (7, 4, 3, and 1%, respectively). 
      When results were compared with the final etiological diagnoses of the patients, 
      the multiplex HV PCR showed high specificity for the diagnosis of CMV and VZV 
      neurological diseases and for cerebral lymphoma (0.95, 0.97, and 0.99, 
      respectively). The sensitivity of the assay was high for CMV disease (0.87), was 
      low for cerebral lymphoma (0.33), and was not evaluable for VZV disease due to 
      the small number of patients with this diagnosis. Nevertheless, detection of VZV 
      DNA had possible diagnostic value in four of the nine cases, and EBV DNA 
      amplification always predicted the diagnosis of cerebral lymphoma in patients 
      with cerebral masses. Detection of HSV DNA was frequently associated with CMV 
      amplification and fatal encephalitis. HHV-6 was not considered to have a 
      pathogenetic role in the three cases in which it was detected. This multiplex HV 
      PCR assay is a specific and clinically useful method for the evaluation of 
      HIV-infected patients with neurological disorders related to HV.
FAU - Quereda, C
AU  - Quereda C
AD  - Unidad de Enfermedades Infecciosas, Hospital Ramon y Cajal, 28034-Madrid, Spain. 
      cqueredar@hrc.insalud.es
FAU - Corral, I
AU  - Corral I
FAU - Laguna, F
AU  - Laguna F
FAU - Valencia, M E
AU  - Valencia ME
FAU - Tenorio, A
AU  - Tenorio A
FAU - Echeverria, J E
AU  - Echeverria JE
FAU - Navas, E
AU  - Navas E
FAU - Martin-Davila, P
AU  - Martin-Davila P
FAU - Moreno, A
AU  - Moreno A
FAU - Moreno, V
AU  - Moreno V
FAU - Gonzalez-Lahoz, J M
AU  - Gonzalez-Lahoz JM
FAU - Arribas, J R
AU  - Arribas JR
FAU - Guerrero, A
AU  - Guerrero A
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (DNA, Viral)
SB  - IM
MH  - AIDS-Related Opportunistic Infections/*diagnosis/virology
MH  - Adult
MH  - Central Nervous System Viral Diseases/*diagnosis/virology
MH  - Cerebrospinal Fluid/virology
MH  - Cytomegalovirus/genetics/isolation & purification
MH  - DNA, Viral/*cerebrospinal fluid
MH  - Female
MH  - Herpesviridae/genetics/*isolation & purification
MH  - Herpesviridae Infections/*diagnosis/virology
MH  - Herpesvirus 3, Human/genetics/isolation & purification
MH  - Herpesvirus 4, Human/genetics/isolation & purification
MH  - Herpesvirus 6, Human/genetics/isolation & purification
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction/*methods
MH  - Reproducibility of Results
MH  - Simplexvirus/genetics/isolation & purification
PMC - PMC87185
EDAT- 2000/08/02 11:00
MHDA- 2000/09/23 11:01
PMCR- 2000/08/01
CRDT- 2000/08/02 11:00
PHST- 2000/08/02 11:00 [pubmed]
PHST- 2000/09/23 11:01 [medline]
PHST- 2000/08/02 11:00 [entrez]
PHST- 2000/08/01 00:00 [pmc-release]
AID - 0138 [pii]
AID - 10.1128/JCM.38.8.3061-3067.2000 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2000 Aug;38(8):3061-7. doi: 10.1128/JCM.38.8.3061-3067.2000.