PMID- 10923299
OWN - NLM
STAT- MEDLINE
DCOM- 20001207
LR  - 20190607
IS  - 0148-0731 (Print)
IS  - 0148-0731 (Linking)
VI  - 122
IP  - 3
DP  - 2000 Jun
TI  - Biological activity of rhBMP-2 released from PLGA microspheres.
PG  - 289-92
AB  - Human recombinant bone morphogenetic protein-2 (rhBMP-2) has been proven 
      effective in stimulating the regeneration of bone in both skeletal and 
      extraskeletal locations. Through encapsulation within, and release from, 
      biodegradable poly(DL-lactic-co-glycolic acid) (PLGA) microspheres, a proven 
      vehicle for sustained delivery of various proteins, the local concentrations of 
      rhBMP-2 could be maintained at optimal levels to stimulate bone regeneration and 
      remodeling at the site of healing in diverse clinical settings. Thus the purpose 
      of this work was to investigate the encapsulation of rhBMP-2 in PLGA microspheres 
      and its biologic activity upon release. Using in vitro tests in simulated body 
      fluids, the effect of rhBMP-2 released from PLGA microspheres upon osteoblast 
      cell cultures was found to be statistically similar to the effect produced by 
      positive controls consisting of nonencapsulated aqueous rhBMP-2 in simulated body 
      fluids. This clarifies an important step in skeletal tissue engineering 
      strategies aimed at the use of encapsulated rhBMP-2 to stimulate bone 
      regeneration and remodeling.
FAU - Oldham, J B
AU  - Oldham JB
AD  - Department of Orthopedic Surgery, Mayo Clinic, Rochester, MN 55905, USA.
FAU - Lu, L
AU  - Lu L
FAU - Zhu, X
AU  - Zhu X
FAU - Porter, B D
AU  - Porter BD
FAU - Hefferan, T E
AU  - Hefferan TE
FAU - Larson, D R
AU  - Larson DR
FAU - Currier, B L
AU  - Currier BL
FAU - Mikos, A G
AU  - Mikos AG
FAU - Yaszemski, M J
AU  - Yaszemski MJ
LA  - eng
GR  - R01-AF44381/AF/ACF HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biomech Eng
JT  - Journal of biomechanical engineering
JID - 7909584
RN  - 0 (BMP2 protein, human)
RN  - 0 (Biocompatible Materials)
RN  - 0 (Bone Morphogenetic Protein 2)
RN  - 0 (Bone Morphogenetic Proteins)
RN  - 0 (Drug Carriers)
RN  - 0 (Polymers)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (recombinant human bone morphogenetic protein-2)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
SB  - IM
MH  - Biocompatible Materials/*therapeutic use
MH  - Bone Morphogenetic Protein 2
MH  - Bone Morphogenetic Proteins/*therapeutic use
MH  - Bone Regeneration/*drug effects
MH  - Bone Remodeling/*drug effects
MH  - Cells, Cultured/drug effects
MH  - Drug Carriers
MH  - Drug Evaluation, Preclinical
MH  - Fetus/cytology
MH  - Humans
MH  - Lactic Acid/*therapeutic use
MH  - *Microspheres
MH  - Osteoblasts/*cytology/*drug effects
MH  - Polyglycolic Acid/*therapeutic use
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Polymers/*therapeutic use
MH  - Recombinant Proteins/*therapeutic use
MH  - *Transforming Growth Factor beta
OTO - NASA
OT  - Non-programmatic
EDAT- 2000/08/03 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/08/03 11:00
PHST- 2000/08/03 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/08/03 11:00 [entrez]
AID - 10.1115/1.429662 [doi]
PST - ppublish
SO  - J Biomech Eng. 2000 Jun;122(3):289-92. doi: 10.1115/1.429662.