PMID- 10923339
OWN - NLM
STAT- MEDLINE
DCOM- 20000912
LR  - 20061115
IS  - 1286-0115 (Print)
IS  - 1286-0115 (Linking)
VI  - 84
IP  - 264
DP  - 2000 Mar
TI  - [Determination, by in situ hybridization on interphasic nucleus, a cytogenetic 
      DNA index; application to breast cancer; comparison of this DNA index to DNA 
      indexes determined by imaging and flow cytometry].
PG  - 41-8
AB  - In oncology, flow cytometry (FCM) and image cytometry (ICM) are commonly used to 
      detect DNA aneuploid cell populations in solid tumors. Agreement between these 
      two approaches is good. The use of both techniques in association minimizes the 
      rate of FCM and ICM false negatives and gives better DNA pattern 
      characterization, particularly for detection of any tumoral component in the FCM 
      DNA diploid peak. Nevertheless, discrepancies exist between the FCM and the ICM 
      DNA index values: the ICM DNA index is often greater than the FCM DNA index. The 
      aim of the present study was to establish a cytogenetic DNA index by determining 
      the chromosomal ploidy using a molecular cytogenetic approach and to compare it 
      to the FCM and ICM DNA indexes. We present here the fluorescence in situ 
      hybridization (FISH) technique we have adapted to the study of breast cancer in 
      order to count the number of copies of the 22 + X human chromosomes in 
      interphasic nuclei. This was achieved using a panel of 21 indirect FITC labeled 
      probes which recognize specific chromosomic DNA sequences. Preliminary results 
      obtained from DNA diploid and DNA aneuploid tumors are discussed.
FAU - Vago, P
AU  - Vago P
AD  - Histologie Embryologie Cytogenetique, Faculte de Medecine de Montpellier-Nimes, 
      France. vago@infobiogen.fr
FAU - Lecart, S
AU  - Lecart S
FAU - Simony, J
AU  - Simony J
FAU - Lequeux, N
AU  - Lequeux N
FAU - Radal, M
AU  - Radal M
FAU - Bureau, J P
AU  - Bureau JP
LA  - fre
PT  - Comparative Study
PT  - English Abstract
PT  - Journal Article
TT  - Determination, par hybridation in situ sur noyau interphasique, d'un index d'ADN 
      cytogenetique; application au cancer du sein; comparaison de cet index d'ADN aux 
      index d'ADN determines par cytometrie en image et en flux.
PL  - France
TA  - Morphologie
JT  - Morphologie : bulletin de l'Association des anatomistes
JID - 9814314
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Breast Neoplasms/*genetics/pathology
MH  - Cell Nucleus/pathology
MH  - DNA, Neoplasm/*analysis
MH  - *Flow Cytometry
MH  - Humans
MH  - *Image Cytometry
MH  - *In Situ Hybridization
MH  - Interphase
MH  - Karyotyping
MH  - Ploidies
EDAT- 2000/08/03 11:00
MHDA- 2000/09/19 11:01
CRDT- 2000/08/03 11:00
PHST- 2000/08/03 11:00 [pubmed]
PHST- 2000/09/19 11:01 [medline]
PHST- 2000/08/03 11:00 [entrez]
PST - ppublish
SO  - Morphologie. 2000 Mar;84(264):41-8.