PMID- 10923620
OWN - NLM
STAT- MEDLINE
DCOM- 20000810
LR  - 20190910
IS  - 0955-3002 (Print)
IS  - 0955-3002 (Linking)
VI  - 76
IP  - 7
DP  - 2000 Jul
TI  - Absence of delayed chromosomal instability in a normal human fibroblast cell line 
      after 125I iododeoxyuridine.
PG  - 963-9
AB  - PURPOSE: To seek the delayed appearance of chromosomal abnormalities in human 
      fibroblasts exposed to the Auger electron emitter 125I. MATERIALS AND METHODS: 
      Normal untransformed human fibroblasts, HF19, were exposed to a concentration of 
      [I125]IUdR, which allowed the survival of 37% of clonogens. Chromosomal analysis 
      using both conventional Giemsa and fluorescence in situ hybridization (FISH) was 
      undertaken on non-clonal bulk cultures from 2 to 39 days after treatment. 
      RESULTS: The data show a declining level of unstable aberrations in the progeny 
      of HF19 fibroblasts exposed to [I125]IUdR, eventually reaching control levels. 
      CONCLUSIONS: The results provide evidence that [125I]IUdR does not induce ongoing 
      chromosomal instability in long-term culture, and gives further support to the 
      use of Auger-electron emitting radionuclides in the treatment and diagnosis of 
      tumours.
FAU - Griffin, C S
AU  - Griffin CS
AD  - Radiation and Genome Stability Unit, MRC, Harwell, Oxon, UK. 
      c.griffin@har.mrc.ac.uk
FAU - Neshasateh-Riz, A
AU  - Neshasateh-Riz A
FAU - Mairs, R J
AU  - Mairs RJ
FAU - Wright, E G
AU  - Wright EG
FAU - Wheldon, T E
AU  - Wheldon TE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Int J Radiat Biol
JT  - International journal of radiation biology
JID - 8809243
RN  - 0 (Iodine Radioisotopes)
RN  - LGP81V5245 (Idoxuridine)
SB  - IM
MH  - Cell Line
MH  - *Chromosome Aberrations
MH  - Fibroblasts/radiation effects
MH  - Humans
MH  - Idoxuridine/toxicity
MH  - In Situ Hybridization, Fluorescence
MH  - Iodine Radioisotopes/*toxicity
EDAT- 2000/08/03 11:00
MHDA- 2000/08/12 11:00
CRDT- 2000/08/03 11:00
PHST- 2000/08/03 11:00 [pubmed]
PHST- 2000/08/12 11:00 [medline]
PHST- 2000/08/03 11:00 [entrez]
AID - 10.1080/09553000050050972 [doi]
PST - ppublish
SO  - Int J Radiat Biol. 2000 Jul;76(7):963-9. doi: 10.1080/09553000050050972.