PMID- 10923916
OWN - NLM
STAT- MEDLINE
DCOM- 20000810
LR  - 20070502
IS  - 0046-8177 (Print)
IS  - 0046-8177 (Linking)
VI  - 31
IP  - 7
DP  - 2000 Jul
TI  - Gene expression of vascular endothelial growth factor in giant cell tumors of 
      bone.
PG  - 804-12
AB  - The production of vascular endothelial growth factors (VEGF), a major cause of 
      neoangiogenesis, is a prerequisite for tumor growth and invasion. VEGF have also 
      been shown to be important for the formation of osteoclasts. Because giant cell 
      tumors of bone (GCT) are frequently hypervascular and have the ability to recruit 
      macrophages and multinucleated osteoclast-like giant cells, we evaluated the 
      levels of VEGF gene transcript in several of these tumors using Northern blot 
      analyses, semiquantitative reverse transcription polymerase chain reaction 
      (RT-PCR), fluorescence in situ hybridization (FISH), and immunohistochemistry. 
      Our results showed that three major isoforms of VEGF (121, 165, and 189) were 
      expressed in all cases of GCT investigated, with isoform 121 transcripts the most 
      abundant. By both FISH and immunohistochemistry, we have shown that VEGF was 
      present in spindle-shaped stromal-like tumor cells, round macrophage-like cells, 
      and osteoclast-like multinucleate giant cells. Moreover, we have shown that the 
      levels of VEGF gene expression but not microvessel density correlated with 
      Enneking's clinical stage of GCT. There were higher levels of VEGF gene 
      expression in stage III GCT than in stage I/II GCT (P < .0357). In conclusion, 
      our results indicate that overexpression of VEGF may be associated with the 
      advanced stage of the neoplasm.
FAU - Zheng, M H
AU  - Zheng MH
AD  - Department of Orthopaedic Surgery, University of Western Australia, Nedlands, 
      Australia.
FAU - Xu, J
AU  - Xu J
FAU - Robbins, P
AU  - Robbins P
FAU - Pavlos, N
AU  - Pavlos N
FAU - Wysocki, S
AU  - Wysocki S
FAU - Kumta, S M
AU  - Kumta SM
FAU - Wood, D J
AU  - Wood DJ
FAU - Papadimitriou, J M
AU  - Papadimitriou JM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Hum Pathol
JT  - Human pathology
JID - 9421547
RN  - 0 (DNA Probes)
RN  - 0 (Endothelial Growth Factors)
RN  - 0 (Lymphokines)
RN  - 0 (RNA, Messenger)
RN  - 0 (VEGFA protein, human)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (Vascular Endothelial Growth Factors)
SB  - IM
MH  - Adult
MH  - Animals
MH  - Blotting, Northern
MH  - Bone Neoplasms/blood supply/*metabolism/pathology
MH  - DNA Probes
MH  - Endothelial Growth Factors/*genetics
MH  - Female
MH  - *Gene Expression
MH  - Giant Cell Tumor of Bone/blood supply/*metabolism/pathology
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphokines/*genetics
MH  - Male
MH  - Mice
MH  - Microcirculation/pathology
MH  - Middle Aged
MH  - Prognosis
MH  - RNA, Messenger/analysis
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Vascular Endothelial Growth Factor A
MH  - Vascular Endothelial Growth Factors
EDAT- 2000/08/03 11:00
MHDA- 2000/08/12 11:00
CRDT- 2000/08/03 11:00
PHST- 2000/08/03 11:00 [pubmed]
PHST- 2000/08/12 11:00 [medline]
PHST- 2000/08/03 11:00 [entrez]
AID - S0046-8177(00)90819-0 [pii]
AID - 10.1053/hupa.2000.8441 [doi]
PST - ppublish
SO  - Hum Pathol. 2000 Jul;31(7):804-12. doi: 10.1053/hupa.2000.8441.