PMID- 10923924
OWN - NLM
STAT- MEDLINE
DCOM- 20000810
LR  - 20061115
IS  - 0046-8177 (Print)
IS  - 0046-8177 (Linking)
VI  - 31
IP  - 7
DP  - 2000 Jul
TI  - Interphase cytogenetics in oncocytic adenomas and carcinomas of the thyroid 
      gland.
PG  - 854-9
AB  - Follicular neoplasms of oncocytic type in the thyroid gland frequently cause 
      diagnostic problems and prognostic uncertainties. To identify numerical 
      chromosomal aberrations of possible pathogenetic importance, we determined 
      chromosome copy numbers in situ in interphase nuclei of 31 oncocytic adenomas and 
      25 oncocytic carcinomas. Archival formaldehyde-fixed, paraffin-embedded tumor 
      samples and normal control thyroid tissues were arranged in arrays and analyzed 
      by fluorescence in situ hybridization (FISH). We used pericentromeric or locus 
      specific probes for chromosomes 1, 7, 8, 9, 11, 12, 17, 18, 22, and X as well as 
      for the oncogenes Her2/neu, cyclin D1, N-myc, and c-myc. The average number of 
      aneusomies per nucleus was significantly higher in carcinomas than in adenomas, 
      and in both, monosomies were more frequent than polysomies. Loss of chromosome 22 
      was found in 8 of 21 (38%) carcinomas; in 5 cases, it was associated with 
      chromosome 2 monosomy. Conversely, chromosome 2 aberrations were not found in 
      adenomas. Monosomies for chromosome 8 and X were detected in most adenomas and 
      carcinomas. The most common gains in adenomas and carcinomas were for chromosome 
      7 (13.8% and 32.0% of the cases, respectively), chromosome 12 (9.6% and 12.0%), 
      and chromosome 17 (19.3% and 32.0%). None of the adenomas with trisomy 17 was 
      associated with gains for chromosomes 7 and 12. None of the analyzed oncogenes 
      was found to be amplified by FISH analysis. Our results indicate that numerical 
      chromosomal aberrations in oncocytic follicular tumors of the thyroid gland are 
      common findings and suggest that different patterns of aberrations may occur in 
      these neoplasms.
FAU - Mazzucchelli, L
AU  - Mazzucchelli L
AD  - Institute of Pathology, University of Bern, Switzerland.
FAU - Burckhardt, E
AU  - Burckhardt E
FAU - Hirsiger, H
AU  - Hirsiger H
FAU - Kappeler, A
AU  - Kappeler A
FAU - Laissue, J A
AU  - Laissue JA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Hum Pathol
JT  - Human pathology
JID - 9421547
RN  - 0 (DNA Probes)
SB  - IM
MH  - Adenoma/*genetics
MH  - Adult
MH  - Aged
MH  - Carcinoma/*genetics
MH  - *Chromosome Aberrations
MH  - *Cytogenetics
MH  - DNA Probes
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - *Interphase
MH  - Male
MH  - Middle Aged
MH  - Oncogenes
MH  - Thyroid Neoplasms/*genetics
EDAT- 2000/08/03 11:00
MHDA- 2000/08/12 11:00
CRDT- 2000/08/03 11:00
PHST- 2000/08/03 11:00 [pubmed]
PHST- 2000/08/12 11:00 [medline]
PHST- 2000/08/03 11:00 [entrez]
AID - S0046-8177(00)91472-2 [pii]
AID - 10.1053/hupa.2000.8444 [doi]
PST - ppublish
SO  - Hum Pathol. 2000 Jul;31(7):854-9. doi: 10.1053/hupa.2000.8444.