PMID- 10927040
OWN - NLM
STAT- MEDLINE
DCOM- 20000830
LR  - 20190707
IS  - 0015-0282 (Print)
IS  - 0015-0282 (Linking)
VI  - 74
IP  - 2
DP  - 2000 Aug
TI  - Role of mitotic control in spermatogenesis.
PG  - 251-6
AB  - OBJECTIVE: To study the correlation between the incidence of sex chromosome 
      aneuploidies in the somatic cells and spermatozoa in karyotypically normal 
      infertile men and fertile donors. DESIGN: A prospective, phase two, controlled 
      study. SETTING: A teaching Hospital Reproductive Medicine and Medical Genetics 
      Units. PATIENT(S): Ten patients with idiopathic oligozoospermia and 10 sperm 
      donors with proven fertility, all with a normal karyotype 46, XY. 
      INTERVENTION(S): Multicolor fluorescence in situ hybridization (FISH) of 
      peripheral blood lymphocytes and spermatozoa using a probe cocktail containing 
      the alpha satellite DXZ1 for the X centromere, DYZ1 for the heterochromatic 
      region of the long arm of the Y, and cosmids D21S259, D21S341, and D21S342 for 
      Down syndrome critical region of chromosome 21. MAIN OUTCOME MEASURE(S): The 
      incidence of chromosome X, Y, and 21 aneuploidies in peripheral lymphocytes and 
      spermatozoa in both groups. RESULT(S): The incidence of aneuploidies related to 
      chromosomes X, Y, and 21 were significantly higher in peripheral lymphocytes and 
      spermatozoa of infertile men compared with donors. There was a positive 
      correlation between the incidence of chromosome aneuploidies in the somatic cells 
      and sperm in all men. CONCLUSION(S): These findings provide suggestive evidence 
      for the importance of mitosis in spermatogenesis and the role of mitotic 
      instability in unexplained oligozoospermia.
FAU - Gazvani, M R
AU  - Gazvani MR
AD  - Department of Obstetrics and Gynaecology, Liverpool Women's Hospital, Liverpool, 
      UK. m.r.gazvani@abdn.ac.uk
FAU - Wilson, E D
AU  - Wilson ED
FAU - Richmond, D H
AU  - Richmond DH
FAU - Howard, P J
AU  - Howard PJ
FAU - Kingsland, C R
AU  - Kingsland CR
FAU - Lewis-Jones, D I
AU  - Lewis-Jones DI
LA  - eng
PT  - Clinical Trial
PT  - Clinical Trial, Phase II
PT  - Controlled Clinical Trial
PT  - Journal Article
PL  - United States
TA  - Fertil Steril
JT  - Fertility and sterility
JID - 0372772
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aneuploidy
MH  - Chromosomes, Human, Pair 21
MH  - Humans
MH  - Infertility, Male/*genetics
MH  - Karyotyping
MH  - Lymphocytes/physiology
MH  - Male
MH  - *Mitosis
MH  - Prospective Studies
MH  - Spermatogenesis/*genetics
MH  - Spermatozoa/physiology
MH  - X Chromosome
MH  - Y Chromosome
EDAT- 2000/08/06 11:00
MHDA- 2000/09/02 11:01
CRDT- 2000/08/06 11:00
PHST- 2000/08/06 11:00 [pubmed]
PHST- 2000/09/02 11:01 [medline]
PHST- 2000/08/06 11:00 [entrez]
AID - S0015-0282(00)00603-8 [pii]
AID - 10.1016/s0015-0282(00)00603-8 [doi]
PST - ppublish
SO  - Fertil Steril. 2000 Aug;74(2):251-6. doi: 10.1016/s0015-0282(00)00603-8.