PMID- 10928998
OWN - NLM
STAT- MEDLINE
DCOM- 20000825
LR  - 20191210
IS  - 0892-6638 (Print)
IS  - 0892-6638 (Linking)
VI  - 14
IP  - 11
DP  - 2000 Aug
TI  - HGF/scatter factor selectively promotes cell invasion by increasing integrin 
      avidity.
PG  - 1629-40
AB  - Hepatocyte growth factor/scatter factor (HGF/SF) controls a genetic program known 
      as 'invasive growth', which involves as critical steps cell adhesion, migration, 
      and trespassing of basement membranes. We show here that in MDA-MB-231 carcinoma 
      cells, these steps are elicited by HGF/SF but not by epidermal growth factor 
      (EGF). Neither factor substantially alters the production or activity of 
      extracellular matrix proteases. HGF/SF, but not EGF, selectively promotes cell 
      adhesion on laminins 1 and 5, fibronectin, and vitronectin through a 
      PI3-K-dependent mechanism. Increased adhesion is followed by enhanced 
      invasiveness through isolated matrix proteins as well as through reconstituted 
      basement membranes. Inhibition assays using function-blocking antibodies show 
      that this phenomenon is mediated by multiple integrins including beta1, beta3, 
      beta4, and beta5. HGF/SF triggers clustering of all these integrins at actin-rich 
      adhesive sites and lamellipodia but does not quantitatively modify their membrane 
      expression. These data suggest that HGF/SF promotes cell adhesion and 
      invasiveness by increasing the avidity of integrins for their specific ligands.
FAU - Trusolino, L
AU  - Trusolino L
AD  - Institute for Cancer Research and Treatment, University of Torino Medical School, 
      10060 Candiolo-Torino, Italy. cboccaccio@ircc.unito.it
FAU - Cavassa, S
AU  - Cavassa S
FAU - Angelini, P
AU  - Angelini P
FAU - Ando, M
AU  - Ando M
FAU - Bertotti, A
AU  - Bertotti A
FAU - Comoglio, P M
AU  - Comoglio PM
FAU - Boccaccio, C
AU  - Boccaccio C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - FASEB J
JT  - FASEB journal : official publication of the Federation of American Societies for 
      Experimental Biology
JID - 8804484
RN  - 0 (Actins)
RN  - 0 (Antibodies)
RN  - 0 (Cadherins)
RN  - 0 (Drug Combinations)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Integrins)
RN  - 0 (Laminin)
RN  - 0 (Ligands)
RN  - 0 (Phosphoinositide-3 Kinase Inhibitors)
RN  - 0 (Proteoglycans)
RN  - 119978-18-6 (matrigel)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - 9007-34-5 (Collagen)
RN  - EC 3.4.21.73 (Urokinase-Type Plasminogen Activator)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Actins/drug effects/metabolism
MH  - Antibodies/immunology/pharmacology
MH  - Basement Membrane/chemistry/metabolism
MH  - Breast Neoplasms/enzymology/*metabolism/*pathology
MH  - Cadherins/metabolism
MH  - Cell Adhesion/drug effects
MH  - Cell Division/drug effects
MH  - Cell Movement/*drug effects
MH  - Collagen/metabolism
MH  - Cytoskeleton/drug effects/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Drug Combinations
MH  - Epidermal Growth Factor/pharmacology
MH  - Extracellular Matrix Proteins/metabolism
MH  - Gene Expression/drug effects
MH  - Hepatocyte Growth Factor/*pharmacology
MH  - Humans
MH  - Integrins/antagonists & inhibitors/immunology/*metabolism
MH  - Laminin/metabolism
MH  - Ligands
MH  - Matrix Metalloproteinase 9/metabolism
MH  - Neoplasm Invasiveness/pathology
MH  - Phenotype
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Phosphoinositide-3 Kinase Inhibitors
MH  - Proteoglycans/metabolism
MH  - Tumor Cells, Cultured
MH  - Urokinase-Type Plasminogen Activator/metabolism
EDAT- 2000/08/06 11:00
MHDA- 2000/09/02 11:01
CRDT- 2000/08/06 11:00
PHST- 2000/08/06 11:00 [pubmed]
PHST- 2000/09/02 11:01 [medline]
PHST- 2000/08/06 11:00 [entrez]
AID - 10.1096/fj.14.11.1629 [doi]
PST - ppublish
SO  - FASEB J. 2000 Aug;14(11):1629-40. doi: 10.1096/fj.14.11.1629.