PMID- 10930208
OWN - NLM
STAT- MEDLINE
DCOM- 20001115
LR  - 20131121
IS  - 0832-610X (Print)
IS  - 0832-610X (Linking)
VI  - 47
IP  - 7
DP  - 2000 Jul
TI  - Milrinone improves intestinal villus blood flow during endotoxemia.
PG  - 673-9
AB  - PURPOSE: To determine whether the compromised intestinal villus blood flow in a 
      rat model of endotoxemia could be improved by continuous infusion of the 
      phosphodiesterase (PDE) inhibitor milrinone. METHODS: Twenty-four anesthetized 
      and ventilated rats were laparotomized and an ileal portion was exteriorized and 
      opened by an antimesenteric incision. The ileal segment was fixed with the 
      mucosal surface upward. Microcirculatory parameters were assessed by intravital 
      videomicroscopy. The animals were randomly assigned to receive one of three 
      treatments: infusion of Escherichia coli lipopolysaccharides without 
      phosphodiesterase inhibitor pretreatment (=LPS group); or infusion of LPS with 
      milrinone pretreatment (= milrinone group), or without infusion of LPS or 
      milrinone (=control group). Macrohemodynamic parameters (MAP, HR) and 
      microhemodynamic parameters of ileal mucosa (mean diameter of central arterioles 
      = D(A) and mean erythrocyte velocity within the arterioles= V(E)) were measured 
      30 min before and at 0, 60, and 120 min after induction of endotoxemia. Mucosal 
      villus blood flow was calculated from D(A) and V(E). RESULTS: In the milrinone 
      group MAP decreased 60 min after induction of endotoxemia whereas it remained 
      stable in the control and the LPS group. In both groups given endotoxin V(E) 
      decreased after start of LPS infusion. In contrast, D(A) decreased in the LPS 
      group, but increased in the milrinone group after 120 min of endotoxemia. Thus, 
      the endotoxin-induced decrease of intestinal villus blood flow was diminished but 
      not fully restored by milrinone infusion. CONCLUSION: Our results indicate that 
      milrinone has some beneficial microcirculatory effects during endotoxemia. 
      Although it contributed to systemic hypotension, it attenuated intestinal mucosal 
      hypoperfusion.
FAU - Schmidt, W
AU  - Schmidt W
AD  - Department of Anesthesiology, University of Heidelberg, Germany. 
      werner.schmidt@urz.uni-heidelberg.de
FAU - Tinelli, M
AU  - Tinelli M
FAU - Secchi, A
AU  - Secchi A
FAU - Gebhard, M M
AU  - Gebhard MM
FAU - Martin, E
AU  - Martin E
FAU - Schmidt, H
AU  - Schmidt H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Can J Anaesth
JT  - Canadian journal of anaesthesia = Journal canadien d'anesthesie
JID - 8701709
RN  - 0 (Cardiotonic Agents)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Phosphodiesterase Inhibitors)
RN  - 0 (Vasodilator Agents)
RN  - JU9YAX04C7 (Milrinone)
SB  - IM
MH  - Animals
MH  - Arterioles/drug effects/physiopathology
MH  - Blood Pressure/drug effects
MH  - Cardiotonic Agents/pharmacology
MH  - Endotoxemia/*physiopathology
MH  - Erythrocytes/drug effects/physiology
MH  - Heart Rate/drug effects
MH  - Ileum/blood supply/drug effects
MH  - Intestinal Mucosa/*blood supply/drug effects
MH  - Lipopolysaccharides
MH  - Male
MH  - Milrinone/*pharmacology
MH  - Phosphodiesterase Inhibitors/*pharmacology
MH  - Rats
MH  - Rats, Wistar
MH  - Regional Blood Flow/drug effects
MH  - Vasodilator Agents/pharmacology
EDAT- 2000/08/10 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/08/10 11:00
PHST- 2000/08/10 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/08/10 11:00 [entrez]
AID - 10.1007/BF03019001 [doi]
PST - ppublish
SO  - Can J Anaesth. 2000 Jul;47(7):673-9. doi: 10.1007/BF03019001.