PMID- 10930987
OWN - NLM
STAT- MEDLINE
DCOM- 20000913
LR  - 20190705
IS  - 0007-1048 (Print)
IS  - 0007-1048 (Linking)
VI  - 110
IP  - 1
DP  - 2000 Jul
TI  - Increased platelet activation and abnormal membrane glycoprotein content and 
      redistribution in myeloproliferative disorders.
PG  - 116-24
AB  - Chronic myeloproliferative disorders (MPDs) are characterized by a high incidence 
      of thrombohaemorrhagic complications, possibly caused by platelet dysfunction. In 
      an attempt to define platelet functional abnormalities, we assessed the 
      expression of activation-dependent membrane proteins in unstimulated and agonist 
      [ADP and thrombin receptor-activating peptide (TRAP)]-stimulated platelets using 
      quantitative whole blood flow cytometry in samples from 50 MPD patients and 30 
      controls. The receptor densities of activation markers and glycoproteins (GPs) 
      were quantified using standardized fluorescent beads. Compared with controls, the 
      mean percentage of P-selectin-positive (15.3% vs. 7.2%; P < 0.001) and 
      thrombospondin (TSP)-positive (6.6% vs. 3.7%; P = 0.003) platelets was increased 
      in unstimulated platelets from patients. Patients having experienced a thrombotic 
      event had a higher mean percentage of TSP-positive non-stimulated platelets than 
      patients without a history of thrombosis (9.0% vs. 4.6%; P = 0.02) and a higher 
      GPIV molecules of equivalent fluorochrome (MEF) value (33113 vs. 24471 MEF; P = 
      0.02). Mean MEF values of monoclonal antibodies (mAbs) against GPIb (34055 vs. 
      38945 MEF; P < 0.001) and GPIIb/IIIa (1416 vs. 1648 MEF; P < 0. 001) were 
      significantly reduced among patients, whereas surface expression of GPIV was 
      increased in patients (28273 vs. 16258 MEF; P < 0.001). In TRAP (10 micromol/l) 
      stimulated whole blood, the MEF of P-selectin (9611 vs. 13293 MEF; P = 0.004) and 
      CD63 (2385 vs. 5177 MEF; P < 0.001) and the ratio of PAC-1/GPIIb/IIIa MEF (0.98 
      vs. 2. 00; P < 0.001) was reduced in patients, indicating either a reduced 
      granule GP content or an intrinsic cellular defect in receptor-mediated granule 
      secretion and activation of the GPIIb/IIIa complex. Expressed as the relative 
      change of MEF compared with unstimulated platelets, TRAP induced decrease of GPIb 
      (7.8% vs. 45%; P < 0.001) and increase of GPIIb/IIIa (49.1% vs. 95.7%; P < 0.001) 
      and GPIV expression (17.8% vs. 55.2%; P < 0.001) was attenuated in patients.
FAU - Jensen, M K
AU  - Jensen MK
AD  - Department of Haematology, Rigshospitalet, University Hospital of Copenhagen, 
      Denmark. mkj@rh.dk
FAU - de Nully Brown, P
AU  - de Nully Brown P
FAU - Lund, B V
AU  - Lund BV
FAU - Nielsen, O J
AU  - Nielsen OJ
FAU - Hasselbalch, H C
AU  - Hasselbalch HC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Br J Haematol
JT  - British journal of haematology
JID - 0372544
RN  - 0 (Bacterial Proteins)
RN  - 0 (Biomarkers)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (MtrB protein, Bacteria)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Transcription Factors)
RN  - 61D2G4IYVH (Adenosine Diphosphate)
SB  - IM
CIN - Br J Haematol. 2001 Jan;112(1):253. PMID: 11225605
MH  - Adenosine Diphosphate
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - *Bacterial Proteins
MH  - Biomarkers/analysis
MH  - Blood Platelets/*metabolism
MH  - Case-Control Studies
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - Male
MH  - Membrane Glycoproteins/*metabolism
MH  - Middle Aged
MH  - Myeloproliferative Disorders/blood/*metabolism
MH  - *Platelet Activation
MH  - RNA-Binding Proteins
MH  - Transcription Factors
EDAT- 2000/08/10 11:00
MHDA- 2000/09/19 11:01
CRDT- 2000/08/10 11:00
PHST- 2000/08/10 11:00 [pubmed]
PHST- 2000/09/19 11:01 [medline]
PHST- 2000/08/10 11:00 [entrez]
AID - bjh2030 [pii]
AID - 10.1046/j.1365-2141.2000.02030.x [doi]
PST - ppublish
SO  - Br J Haematol. 2000 Jul;110(1):116-24. doi: 10.1046/j.1365-2141.2000.02030.x.