PMID- 10932006
OWN - NLM
STAT- MEDLINE
DCOM- 20000925
LR  - 20061115
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 17
IP  - 4
DP  - 2000 Aug
TI  - [Study on interphase cytogenetic abnormalities in malignant cells in pleural 
      fluids from lung cancer cases].
PG  - 244-7
AB  - OBJECTIVE: To study the interphase cytogenetic abnormalities in malignant cells 
      in pleural fluids from lung cancer cases by fluorescence in situ 
      hybridization(FISH) and compare the result of FISH with that of conventional 
      cytology. METHODS: Twenty-six pleural fluids from the lung cancer cases were 
      detected by dual-fluorescence in situ hybridization centromere DNA probes of 
      chromosomes 7, 11, 17, and X. RESULTS: In 19 positive pleural fluids the rates of 
      hyperdiploid in chromosomes 7, X, 17 and 11 were 16(84.2%), 14(73. 7%), 12(63.2%) 
      and 8(42.1%), respectively. In 2 suspicious malignant pleural fluids, both 
      chromosomes 7 and X showed hyperdiploid. Two cases of hyperplasia from 5 negative 
      specimens also showed gain of chromosome X. Another 3 negative pleural fluids had 
      a normal number of chromosome. CONCLUSION: One of the important abnormalities 
      occurred in tumor cells derived from pleural fluid of lung cancer is 
      hyperdiploid, which can be detected by FISH. Therefore, hyperdiploid in pleural 
      fluid cells can be an implication for the malignancy that has not been detected 
      or confirmed by cytological examination.
FAU - Jia, D
AU  - Jia D
AD  - Department of Cytology, Cancer Institute/Hospital, Chinese Academy of Medical 
      Sciences, Beijing, P. R. China. jiadh@yeah.net
FAU - Zhang, Z
AU  - Zhang Z
FAU - Liu, S
AU  - Liu S
FAU - Cheng, S
AU  - Cheng S
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
SB  - IM
MH  - *Chromosome Aberrations
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - Lung Neoplasms/*genetics
MH  - Male
MH  - Pleural Effusion, Malignant/*pathology
MH  - Ploidies
EDAT- 2000/08/10 11:00
MHDA- 2000/09/30 11:01
CRDT- 2000/08/10 11:00
PHST- 2000/08/10 11:00 [pubmed]
PHST- 2000/09/30 11:01 [medline]
PHST- 2000/08/10 11:00 [entrez]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2000 Aug;17(4):244-7.