PMID- 10932015
OWN - NLM
STAT- MEDLINE
DCOM- 20000925
LR  - 20181130
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 17
IP  - 4
DP  - 2000 Aug
TI  - [Detection of epidermal growth factor receptor gene amplification in human 
      laryngeal carcinomas by means of fluorescence in situ hybridization].
PG  - 278-80
AB  - OBJECTIVE: To detect epidermal growth factor receptor(EGFR) gene amplification in 
      human laryngeal carcinoma Hep-2 cell line and laryngeal carcinoma tissues. 
      METHODS: The technique of fluorescence in situ hybridization(FISH). RESULTS: The 
      EGFR gene amplification of a laryngeal carcinoma Hep-2 cell line and 5 laryngeal 
      carcinoma tissues were detected by FISH. In the metaphase chromosome and 
      interphase nuclei of Hep-2 cell line and 2 laryngeal carcinoma tissues, distinct 
      cluster and multiple dot signals were found. In the interphase nuclei of the 
      other 3 laryngeal carcinoma tissues, no increase in the number or extent of the 
      hybrid signals was found. CONCLUSION: Compared with normal diploid cell, the EGFR 
      gene amplification was observed at different levels ranging from 2 to 8 folds in 
      metaphase chromosome and interphase nuclei of Hep-2 cell line and 2 laryngeal 
      carcinoma tissues while no amplification was observed in the other 3 laryngeal 
      carcinoma tissues. The results demonstrate that quantitative detection of 
      amplified gene by FISH in the metaphase chromosome and interphase nuclei is 
      useful for detection of laryngeal carcinoma tissues.
FAU - Li, F
AU  - Li F
AD  - Department of Medical Genetics, China Medical University, Shenyang, Liaoning, P. 
      R. China. klsun@mail.cmu.edu.cn
FAU - Kang, N
AU  - Kang N
FAU - Fu, W
AU  - Fu W
FAU - Sun, X
AU  - Sun X
FAU - Gao, H
AU  - Gao H
FAU - Sun, K
AU  - Sun K
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - ErbB Receptors/*genetics
MH  - *Gene Amplification
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Laryngeal Neoplasms/*genetics
EDAT- 2000/08/10 11:00
MHDA- 2000/09/30 11:01
CRDT- 2000/08/10 11:00
PHST- 2000/08/10 11:00 [pubmed]
PHST- 2000/09/30 11:01 [medline]
PHST- 2000/08/10 11:00 [entrez]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2000 Aug;17(4):278-80.