PMID- 10932853
OWN - NLM
STAT- MEDLINE
DCOM- 20000830
LR  - 20190822
IS  - 0003-2654 (Print)
IS  - 0003-2654 (Linking)
VI  - 125
IP  - 6
DP  - 2000 Jun
TI  - High-performance liquid chromatographic assay of erythromycin from biological 
      matrix using electrochemical or ultraviolet detection.
PG  - 1077-81
AB  - Two chromatographic methods were developed for the determination of erythromycin 
      A (EA) residues in animal tissues (muscle, liver, kidney and fat of cattle, pigs 
      and poultry) and cow's milk. In addition to a more traditional method using 
      electrochemical detection, we developed an original alternative method based on 
      UV detection at 236 nm, by pretreating to create a chromophore in the molecule. 
      An internal standard was used with both methods to check the variability of the 
      analytical system. Analysis times and performance were compared. The recovery of 
      EA from various matrices was greater than 95%. For both methods the 
      quantification limit for EA was 0.25 microgram ml-1 for plasma, 0.025 microgram 
      g-1 for milk and 0.125 microgram g-1 for the other biological matrices. The 
      methods can be used to check for EA residues in these matrices; in fact, the 
      statutory maximum residue limits (MRLs) of EA are 0.4 microgram g-1 in muscle, 
      kidney, liver and fat of beef cattle, sheep, pigs and poultry, and 0.04 microgram 
      g-1 in cow's and sheep's milk.
FAU - Dreassi, E
AU  - Dreassi E
AD  - Dipartimento Farmaco chimico Tecnologico, Siena University, Italy.
FAU - Corti, P
AU  - Corti P
FAU - Bezzini, F
AU  - Bezzini F
FAU - Furlanetto, S
AU  - Furlanetto S
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Analyst
JT  - The Analyst
JID - 0372652
RN  - 0 (Veterinary Drugs)
RN  - 63937KV33D (Erythromycin)
SB  - IM
MH  - Adipose Tissue/chemistry
MH  - Animals
MH  - Cattle
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Drug Residues/*analysis
MH  - Electrochemistry
MH  - Erythromycin/*analysis
MH  - Kidney/chemistry
MH  - Liver/chemistry
MH  - *Meat
MH  - Milk/*chemistry
MH  - Muscles/chemistry
MH  - Poultry
MH  - Sheep
MH  - Spectrophotometry, Ultraviolet
MH  - Swine
MH  - Veterinary Drugs/*analysis
EDAT- 2000/08/10 11:00
MHDA- 2000/09/02 11:01
CRDT- 2000/08/10 11:00
PHST- 2000/08/10 11:00 [pubmed]
PHST- 2000/09/02 11:01 [medline]
PHST- 2000/08/10 11:00 [entrez]
AID - 10.1039/a909876c [doi]
PST - ppublish
SO  - Analyst. 2000 Jun;125(6):1077-81. doi: 10.1039/a909876c.