PMID- 10935505
OWN - NLM
STAT- MEDLINE
DCOM- 20000817
LR  - 20190607
IS  - 1522-8002 (Print)
IS  - 1476-5586 (Electronic)
IS  - 1476-5586 (Linking)
VI  - 2
IP  - 3
DP  - 2000 May-Jun
TI  - Mapping of the gene for the human telomerase reverse transcriptase, hTERT, to 
      chromosome 5p15.33 by fluorescence in situ hybridization.
PG  - 197-201
AB  - Telomerase, the enzyme that maintains the ends of chromosomes, is absent from the 
      majority of somatic cells but is present and active in most tumours. The gene for 
      the reverse transcriptase component of telomerase (hTERT) has recently been 
      identified. A cDNA clone of this gene was used as a probe to identify three 
      genomic bacterial artificial chromosome (BAC) clones, one of which was used as a 
      probe to map hTERT by fluorescence in situ hybridization (FISH) to chromosome 
      5p15.33. This BAC probe was further used to look at copy number of the hTERT 
      region in immortal cell lines. We found that 10/15 immortal cell lines had a 
      modal copy number of 3 or more per cell, with one cell line (CaSki) having a 
      modal copy number of 11. This suggests that increases in copy number of the hTERT 
      gene region do occur, and may well be one route to upregulating telomerase levels 
      in tumour cells. 5p15 gains and amplifications have been documented for various 
      tumour types, including non-small cell lung carcinoma, squamous cell carcinoma of 
      head and neck, and uterine cervix cancer, making hTERT a potential target.
FAU - Bryce, L A
AU  - Bryce LA
AD  - CRC Department of Medical Oncology, University of Glasgow, UK.
FAU - Morrison, N
AU  - Morrison N
FAU - Hoare, S F
AU  - Hoare SF
FAU - Muir, S
AU  - Muir S
FAU - Keith, W N
AU  - Keith WN
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Neoplasia
JT  - Neoplasia (New York, N.Y.)
JID - 100886622
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (telomerase RNA)
RN  - 63231-63-0 (RNA)
RN  - EC 2.7.7.49 (Telomerase)
SB  - IM
CIN - Neoplasia. 2000 May-Jun;2(3):195-6. PMID: 10935504
MH  - *Chromosome Mapping
MH  - *Chromosomes, Human, Pair 5
MH  - DNA-Binding Proteins
MH  - Gene Dosage
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - *RNA
MH  - Telomerase/*genetics
MH  - Tumor Cells, Cultured
PMC - PMC1507564
EDAT- 2000/08/10 11:00
MHDA- 2000/08/19 11:00
PMCR- 2000/05/01
CRDT- 2000/08/10 11:00
PHST- 2000/08/10 11:00 [pubmed]
PHST- 2000/08/19 11:00 [medline]
PHST- 2000/08/10 11:00 [entrez]
PHST- 2000/05/01 00:00 [pmc-release]
AID - 0092a [pii]
AID - 10.1038/sj.neo.7900092 [doi]
PST - ppublish
SO  - Neoplasia. 2000 May-Jun;2(3):197-201. doi: 10.1038/sj.neo.7900092.