PMID- 10936091
OWN - NLM
STAT- MEDLINE
DCOM- 20000925
LR  - 20071115
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 274
IP  - 1
DP  - 2000 Aug 15
TI  - The HTLV-I orfI protein is recognized by serum antibodies from naturally infected 
      humans and experimentally infected rabbits.
PG  - 86-93
AB  - The mechanism of T-cell transformation by human T-cell lymphotropic virus type I 
      (HTLV-I), though not completely understood, appears to involve the interactions 
      of several viral and cellular proteins. One of these viral proteins, p12(I), 
      encoded by HTLV-I orfI, is a weak oncogene that binds the 16-kDa subunit of the 
      vacuolar ATPase and interacts with the immature beta and gamma(c) chains of the 
      IL-2 receptor. We have expressed the singly spliced orfI cDNA in the baculovirus 
      system and used the recombinant protein as a tool to assess the presence of 
      antibodies in naturally or experimentally infected hosts. In addition, rabbit 
      antisera were raised against various p12(I) synthetic peptides and used to 
      identify three antigenic regions within p12(I), one between the two putative 
      transmembrane regions of p12(I) and two at the carboxy-terminus of the protein. 
      More importantly, sera from a naturally infected human (1 of 32) and 
      experimentally infected rabbits (9 of 20) recognized the rp12(I), demonstrating 
      orfI expression and immunogenicity in vivo. Taken together these data provide the 
      first evidence of orfI expression during HTLV-I infections.
CI  - Copyright 2000 Academic Press.
FAU - Dekaban, G A
AU  - Dekaban GA
AD  - Gene Therapy and Molecular Virology Group, The John C. Roberts ResearchInstitute, 
      London, Ontario N6A 5K8, Canada.
FAU - Peters, A A
AU  - Peters AA
FAU - Mulloy, J C
AU  - Mulloy JC
FAU - Johnson, J M
AU  - Johnson JM
FAU - Trovato, R
AU  - Trovato R
FAU - Rivadeneira, E
AU  - Rivadeneira E
FAU - Franchini, G
AU  - Franchini G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Deltaretrovirus Antibodies)
RN  - 0 (Deltaretrovirus Antigens)
RN  - 0 (Oncogene Proteins, Viral)
RN  - 0 (Peptides)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (Vaccines, Synthetic)
RN  - 0 (Viral Regulatory and Accessory Proteins)
RN  - 0 (p12I protein, Human T-lymphotropic virus 1)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cell Line
MH  - Deltaretrovirus Antibodies/blood/*immunology
MH  - Deltaretrovirus Antigens/genetics/*immunology
MH  - Disease Models, Animal
MH  - HTLV-I Infections/blood/immunology
MH  - HeLa Cells
MH  - Human T-lymphotropic virus 1/genetics/*immunology
MH  - Humans
MH  - Molecular Sequence Data
MH  - Oncogene Proteins, Viral/genetics/*immunology
MH  - Peptides/immunology
MH  - Precipitin Tests
MH  - Rabbits
MH  - Recombinant Fusion Proteins/genetics/immunology
MH  - Spodoptera/cytology
MH  - *Transcription Factors
MH  - Vaccines, Synthetic/genetics/immunology
MH  - Viral Regulatory and Accessory Proteins
EDAT- 2000/08/11 11:00
MHDA- 2000/09/30 11:01
CRDT- 2000/08/11 11:00
PHST- 2000/08/11 11:00 [pubmed]
PHST- 2000/09/30 11:01 [medline]
PHST- 2000/08/11 11:00 [entrez]
AID - S0042-6822(00)90406-9 [pii]
AID - 10.1006/viro.2000.0406 [doi]
PST - ppublish
SO  - Virology. 2000 Aug 15;274(1):86-93. doi: 10.1006/viro.2000.0406.