PMID- 10938123
OWN - NLM
STAT- MEDLINE
DCOM- 20000921
LR  - 20240315
IS  - 0270-7306 (Print)
IS  - 1098-5549 (Electronic)
IS  - 0270-7306 (Linking)
VI  - 20
IP  - 17
DP  - 2000 Sep
TI  - Structure and dynamic properties of a glucocorticoid receptor-induced chromatin 
      transition.
PG  - 6466-75
AB  - Activation of the mouse mammary tumor virus (MMTV) promoter by the glucocorticoid 
      receptor (GR) is associated with a chromatin structural transition in the B 
      nucleosome region of the viral long terminal repeat (LTR). Recent evidence 
      indicates that this transition extends upstream of the B nucleosome, encompassing 
      a region larger than a single nucleosome (G. Fragoso, W. D. Pennie, S. John, and 
      G. L. Hager, Mol. Cell. Biol. 18:3633-3644). We have reconstituted MMTV LTR DNA 
      into a polynucleosome array using Drosophila embryo extracts. We show binding of 
      purified GR to specific GR elements within a large, multinucleosome array and 
      describe a GR-induced nucleoprotein transition that is dependent on ATP and a 
      HeLa nuclear extract. Previously uncharacterized GR binding sites in the upstream 
      C nucleosome region are involved in the extended region of chromatin remodeling. 
      We also show that GR-dependent chromatin remodeling is a multistep process; in 
      the absence of ATP, GR binds to multiple sites on the chromatin array and 
      prevents restriction enzyme access to recognition sites. Upon addition of ATP, GR 
      induces remodeling and a large increase in access to enzymes sites within the 
      transition region. These findings suggest a dynamic model in which GR first binds 
      to chromatin after ligand activation, recruits a remodeling activity, and is then 
      lost from the template. This model is consistent with the recent description of a 
      "hit-and-run" mechanism for GR action in living cells (J. G. McNally, W. G. 
      Muller, D. Walker, and G. L. Hager, Science 287:1262-1264, 2000).
FAU - Fletcher, T M
AU  - Fletcher TM
AD  - Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, 
      National Institutes of Health, Bethesda, Maryland 20892-5055, USA.
FAU - Ryu, B W
AU  - Ryu BW
FAU - Baumann, C T
AU  - Baumann CT
FAU - Warren, B S
AU  - Warren BS
FAU - Fragoso, G
AU  - Fragoso G
FAU - John, S
AU  - John S
FAU - Hager, G L
AU  - Hager GL
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (Chromatin)
RN  - 0 (Ligands)
RN  - 0 (Nucleosomes)
RN  - 0 (Receptors, Glucocorticoid)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - EC 3.1.21.1 (Deoxyribonuclease I)
SB  - IM
MH  - 3T3 Cells
MH  - Adenosine Triphosphate/metabolism
MH  - Animals
MH  - Binding Sites
MH  - CHO Cells
MH  - Cell Nucleus/metabolism
MH  - Chromatin/genetics/*metabolism
MH  - Cricetinae
MH  - Deoxyribonuclease I/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Electrophoresis, Agar Gel
MH  - HeLa Cells
MH  - Humans
MH  - Hydrolysis
MH  - Ligands
MH  - Mammary Tumor Virus, Mouse/genetics
MH  - Mice
MH  - Mutagenesis, Site-Directed
MH  - Nucleosomes/metabolism
MH  - Plasmids/metabolism
MH  - Protein Binding
MH  - Receptors, Glucocorticoid/genetics/*metabolism
MH  - Terminal Repeat Sequences
MH  - Transfection
PMC - PMC86121
EDAT- 2000/08/11 11:00
MHDA- 2000/09/23 11:01
PMCR- 2000/09/01
CRDT- 2000/08/11 11:00
PHST- 2000/08/11 11:00 [pubmed]
PHST- 2000/09/23 11:01 [medline]
PHST- 2000/08/11 11:00 [entrez]
PHST- 2000/09/01 00:00 [pmc-release]
AID - 0481 [pii]
AID - 10.1128/MCB.20.17.6466-6475.2000 [doi]
PST - ppublish
SO  - Mol Cell Biol. 2000 Sep;20(17):6466-75. doi: 10.1128/MCB.20.17.6466-6475.2000.