PMID- 10940479
OWN - NLM
STAT- MEDLINE
DCOM- 20001107
LR  - 20190813
IS  - 0303-7207 (Print)
IS  - 0303-7207 (Linking)
VI  - 165
IP  - 1-2
DP  - 2000 Jul 25
TI  - Role of Ets2 in cyclic AMP regulation of the human chorionic gonadotropin beta 
      promoter.
PG  - 17-24
AB  - Activation of the human chorionic gonadotropin beta (hCGbeta) by the protein 
      kinase A (PKA) pathway has been shown to occur through an activating protein-2 
      (AP-2)-dependent mechanism. However, in HepG2 cells, which are deficient in AP-2, 
      the PKA catalytic subunit is still able to stimulate the hCGbeta promoter. Ets2 
      plays a critical role in placental development as revealed by placental 
      abnormalities in Ets2 knockout mice. Transfection of Ets2 into JEG-3 placental 
      cells causes a slight, but reproducible, increase in hCGbeta promoter basal 
      activity. However, cotransfection with the PKA catalytic subunit causes a strong 
      synergistic increase in hCGbeta promoter activity. Ets2 synergistic activation of 
      the hCGbeta promoter is specific for the PKA pathway, as activation of the ras 
      pathway, which also acts through Ets2, does not activate the hCGbeta promoter. 
      c-Jun-mediated repression of hCGbeta is inhibited by Ets2 cotransfection, 
      indicating that protein-protein interactions may be responsible for Ets2 
      activation of the hCGbeta promoter.
FAU - Johnson, W
AU  - Johnson W
AD  - Division of Endocrinology, Metabolism and Molecular Medicine, Northwestern 
      University Medical School, Tarry Building 15-709, 303 East Chicago Avenue, 
      Chicago, IL 60611, USA.
FAU - Jameson, J L
AU  - Jameson JL
LA  - eng
GR  - HD23519/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Ireland
TA  - Mol Cell Endocrinol
JT  - Molecular and cellular endocrinology
JID - 7500844
RN  - 0 (Chorionic Gonadotropin, beta Subunit, Human)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (ERF protein, human)
RN  - 0 (ETS2 protein, human)
RN  - 0 (Ets2 protein, mouse)
RN  - 0 (Proto-Oncogene Protein c-ets-2)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Proto-Oncogene Proteins c-jun)
RN  - 0 (Repressor Proteins)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factor AP-2)
RN  - 0 (Transcription Factors)
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Chorionic Gonadotropin, beta Subunit, Human/*genetics
MH  - Cyclic AMP/*metabolism
MH  - Cyclic AMP-Dependent Protein Kinases/metabolism
MH  - DNA-Binding Proteins/metabolism
MH  - Gene Expression Regulation
MH  - Genes, Reporter
MH  - Genes, ras
MH  - Humans
MH  - Mice
MH  - *Promoter Regions, Genetic
MH  - Proto-Oncogene Protein c-ets-2
MH  - Proto-Oncogene Proteins/genetics/*metabolism
MH  - Proto-Oncogene Proteins c-jun/metabolism
MH  - *Repressor Proteins
MH  - Trans-Activators/genetics/*metabolism
MH  - Transcription Factor AP-2
MH  - Transcription Factors/metabolism
MH  - Transfection
EDAT- 2000/08/15 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/08/15 11:00
PHST- 2000/08/15 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/08/15 11:00 [entrez]
AID - S0303-7207(00)00269-0 [pii]
AID - 10.1016/s0303-7207(00)00269-0 [doi]
PST - ppublish
SO  - Mol Cell Endocrinol. 2000 Jul 25;165(1-2):17-24. doi: 
      10.1016/s0303-7207(00)00269-0.