PMID- 10941340
OWN - NLM
STAT- MEDLINE
DCOM- 20000823
LR  - 20041117
IS  - 0002-9173 (Print)
IS  - 0002-9173 (Linking)
VI  - 114
IP  - 2
DP  - 2000 Aug
TI  - Detection of chromosome 11q13 breakpoints by interphase fluorescence in situ 
      hybridization. A useful ancillary method for the diagnosis of mantle cell 
      lymphoma.
PG  - 248-57
AB  - We assessed cytologic specimens from 11 mantle cell lymphomas (MCLs) and 32 other 
      B-cell non-Hodgkin lymphomas (NHLs) for 11q13 breakpoints using a 2-color 
      fluorescence in situ hybridization (FISH) assay that uses an 11q13 probe centered 
      on the CCND1 gene and a centromeric chromosome 11 probe (CEP11). The number of 
      nuclei in 200 cells were counted, and results were expressed as an 11q13/CEP11 
      ratio. All MCLs showed a high percentage of interphase nuclei with 3 or more 
      11q13 signals (mean, 74.8%; range 57%-90%). In contrast, in other B-cell NHLs the 
      mean percentage of cells with 3 or more 11q13 signals was 9.2%. All MCLs had an 
      elevated 11q13/CEP11 ratio (mean, 1.38). The mean ratio for other B-cell NHLs was 
      0.99. Two non-MCL cases, 1 large B-cell and 1 B-cell unclassified NHL, had high 
      11q13/CEP11 ratios of 1.15 and 1.30, respectively. Conventional cytogenetic 
      analysis performed on the former case revealed a t(5;11)(q31;q13). Interphase 
      FISH analysis using 11q13 and CEP11 probes is a convenient ancillary method for 
      assisting in the diagnosis of MCL. This commercially available assay is simple to 
      use on cytology or imprint specimens, and results can be obtained within 24 
      hours.
FAU - Katz, R L
AU  - Katz RL
AD  - Department of Pathology, University of Texas M.D. Anderson Cancer Center, Houston 
      77030, USA.
FAU - Caraway, N P
AU  - Caraway NP
FAU - Gu, J
AU  - Gu J
FAU - Jiang, F
AU  - Jiang F
FAU - Pasco-Miller, L A
AU  - Pasco-Miller LA
FAU - Glassman, A B
AU  - Glassman AB
FAU - Luthra, R
AU  - Luthra R
FAU - Hayes, K J
AU  - Hayes KJ
FAU - Romaguera, J E
AU  - Romaguera JE
FAU - Cabanillas, F F
AU  - Cabanillas FF
FAU - Medeiros, L J
AU  - Medeiros LJ
LA  - eng
PT  - Journal Article
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
RN  - 0 (Antigens, CD)
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Neoplasm)
RN  - 136601-57-5 (Cyclin D1)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Antigens, CD/analysis
MH  - Cell Nucleus/genetics
MH  - Chromosome Breakage/*genetics
MH  - Chromosome Fragility/*genetics
MH  - Chromosomes, Human, Pair 11/*genetics
MH  - Chromosomes, Human, Pair 14/genetics
MH  - Cyclin D1/analysis
MH  - DNA Probes
MH  - DNA, Neoplasm/analysis
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - Immunophenotyping
MH  - *In Situ Hybridization, Fluorescence
MH  - Interphase/genetics
MH  - Karyotyping
MH  - Lymphoma, B-Cell/chemistry/diagnosis/genetics/immunology
MH  - Lymphoma, Mantle-Cell/chemistry/diagnosis/*genetics/immunology
MH  - Male
MH  - Middle Aged
EDAT- 2000/08/15 11:00
MHDA- 2000/08/29 11:01
CRDT- 2000/08/15 11:00
PHST- 2000/08/15 11:00 [pubmed]
PHST- 2000/08/29 11:01 [medline]
PHST- 2000/08/15 11:00 [entrez]
AID - 10.1309/69EJ-RFM5-E976-BUTP [doi]
PST - ppublish
SO  - Am J Clin Pathol. 2000 Aug;114(2):248-57. doi: 10.1309/69EJ-RFM5-E976-BUTP.