PMID- 10942374
OWN - NLM
STAT- MEDLINE
DCOM- 20000907
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 96
IP  - 4
DP  - 2000 Aug 15
TI  - Dendritic cells transduced by multiply deleted HIV-1 vectors exhibit normal 
      phenotypes and functions and elicit an HIV-specific cytotoxic T-lymphocyte 
      response in vitro.
PG  - 1327-33
AB  - Dendritic cells (DCs) genetically modified to continually express and present 
      antigens may be potent physiologic adjuvants for induction of prophylactic or 
      therapeutic immunity. We have previously shown that an env and nef deleted HIV-1 
      vector (HIV-1 delta EN) pseudotyped with VSV-G transduced monocyte-derived 
      macrophages as well as CD34(+) precursors of DCs. Here we extended these findings 
      with HIV-1 delta EN to highly differentiated human DCs derived in culture from 
      circulating monocytes (DCs). In addition, a new vector derived from HIV-1 delta 
      EN but further deleted in its remaining accessory genes vif, vpr, and vpu (HIV-1 
      delta EN V(3)) was also tested. Both vectors efficiently transduced DCs. 
      Transduction of DCs did not significantly alter their viability or their 
      immunophenotype when compared with untransduced DCs. Furthermore, the phagocytic 
      potential of immature DCs, as well as their ability to differentiate into mature 
      DCs capable of stimulating T-cell proliferation, was not affected. Finally, DCs 
      transduced by the HIV-1 delta EN vector were able to elicit a primary antiviral 
      cytotoxic T-cell response in autologous CD8 T cells. These results suggest that 
      HIV-1-based vectors expressing viral antigens may be useful for in vivo active 
      immunization as well as ex vivo priming of cytotoxic T cells for adoptive T-cell 
      therapy. (Blood. 2000;96:1327-1333)
FAU - Gruber, A
AU  - Gruber A
AD  - Departments of Medicine and Biology, University of California, San Diego, CA, 
      USA.
FAU - Kan-Mitchell, J
AU  - Kan-Mitchell J
FAU - Kuhen, K L
AU  - Kuhen KL
FAU - Mukai, T
AU  - Mukai T
FAU - Wong-Staal, F
AU  - Wong-Staal F
LA  - eng
GR  - AI44372/AI/NIAID NIH HHS/United States
GR  - AI45992/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
SB  - IM
MH  - Acquired Immunodeficiency Syndrome/genetics/immunology/prevention & control
MH  - Antigen Presentation
MH  - CD8-Positive T-Lymphocytes/*immunology
MH  - Cells, Cultured
MH  - Cytotoxicity, Immunologic/*genetics
MH  - Dendritic Cells/*immunology
MH  - Gene Transfer Techniques
MH  - *Genetic Therapy
MH  - *Genetic Vectors
MH  - HIV-1/*genetics
MH  - Humans
MH  - Immunotherapy
EDAT- 2000/08/15 11:00
MHDA- 2000/09/09 11:01
CRDT- 2000/08/15 11:00
PHST- 2000/08/15 11:00 [pubmed]
PHST- 2000/09/09 11:01 [medline]
PHST- 2000/08/15 11:00 [entrez]
AID - S0006-4971(20)71946-4 [pii]
PST - ppublish
SO  - Blood. 2000 Aug 15;96(4):1327-33.