PMID- 10944441
OWN - NLM
STAT- MEDLINE
DCOM- 20000921
LR  - 20061115
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 275
IP  - 1
DP  - 2000 Aug 18
TI  - Cancer cell-derived interleukin 1alpha contributes to autocrine and paracrine 
      induction of pro-metastatic genes in breast cancer.
PG  - 60-2
AB  - Invasion and metastasis of cancer cells is a complex process requiring the 
      activity of proteins that promote extracellular matrix degradation, motility of 
      cancer cells, and angiogenesis. Although exclusively the cancer cells make 
      several of these proteins, few key proteins are derived from stromal cells in 
      response to cancer cell-stromal cell interaction. In this report, we show that 
      the breast cancer cell-derived interleukin-1alpha (IL-1alpha) plays an important 
      role in expression of pro-metastatic genes in cancer as well as in stromal cells. 
      Neutralizing antibody against IL-1alpha inhibited IL-6, and IL-8 expression in 
      IL-1alpha-expressing cancer cells. In addition, this antibody also prevented 
      induction of IL-6, IL-8, and matrix metalloproteinase 3 (MMP3) but not vascular 
      endothelial growth factor (VEGF) in fibroblasts by conditioned medium (CM) from 
      IL-1alpha-expressing breast cancer cells. These results suggest that inhibition 
      of IL-1alpha activity by either neutralizing antibody against IL-1alpha or 
      chemical inhibitor of IL-1alpha processing may prevent invasion and metastasis of 
      breast cancer.
CI  - Copyright 2000 Academic Press.
FAU - Nozaki, S
AU  - Nozaki S
AD  - Department of Medicine, Indiana University School of Medicine, Indianapolis, 
      Indiana, 46202, USA.
FAU - Sledge, G W Jr
AU  - Sledge GW Jr
FAU - Nakshatri, H
AU  - Nakshatri H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Antibodies)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Endothelial Growth Factors)
RN  - 0 (Growth Inhibitors)
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-6)
RN  - 0 (Interleukin-8)
RN  - 0 (LIF protein, human)
RN  - 0 (Leukemia Inhibitory Factor)
RN  - 0 (Lymphokines)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (Vascular Endothelial Growth Factors)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Antibodies/immunology/pharmacology
MH  - Autocrine Communication/*drug effects
MH  - Breast Neoplasms/*genetics/metabolism/*pathology
MH  - Culture Media, Conditioned/pharmacology
MH  - Endothelial Growth Factors/genetics
MH  - Fibroblasts/drug effects/metabolism
MH  - Gene Expression Regulation, Neoplastic/*drug effects
MH  - Growth Inhibitors/antagonists & inhibitors/immunology/pharmacology
MH  - Humans
MH  - Interleukin-1/antagonists & inhibitors/immunology/*pharmacology
MH  - Interleukin-6/genetics
MH  - Interleukin-8/genetics
MH  - Leukemia Inhibitory Factor
MH  - Lymphokines/antagonists & inhibitors/genetics/immunology/pharmacology
MH  - Matrix Metalloproteinase 3/genetics
MH  - Neoplasm Invasiveness/genetics
MH  - Neoplasm Metastasis/genetics
MH  - Neoplasm Proteins/genetics
MH  - Paracrine Communication/*drug effects
MH  - RNA, Messenger/metabolism
MH  - Tumor Cells, Cultured
MH  - Vascular Endothelial Growth Factor A
MH  - Vascular Endothelial Growth Factors
EDAT- 2000/08/17 11:00
MHDA- 2000/09/23 11:01
CRDT- 2000/08/17 11:00
PHST- 2000/08/17 11:00 [pubmed]
PHST- 2000/09/23 11:01 [medline]
PHST- 2000/08/17 11:00 [entrez]
AID - S0006-291X(00)93241-8 [pii]
AID - 10.1006/bbrc.2000.3241 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2000 Aug 18;275(1):60-2. doi: 10.1006/bbrc.2000.3241.