PMID- 10945228
OWN - NLM
STAT- MEDLINE
DCOM- 20000816
LR  - 20131121
IS  - 1044-5498 (Print)
IS  - 1044-5498 (Linking)
VI  - 19
IP  - 7
DP  - 2000 Jul
TI  - Specific cell targeting for delivery of toxins into small-cell lung cancer using 
      a streptavidin fusion protein complex.
PG  - 383-8
AB  - New modalities of treatment for small-cell lung cancer (SCLC) are needed, because 
      the majority of patients continue to die of disseminated disease despite an 
      initial response to conventional chemotherapy. Abnormal surface expression of the 
      neural-cell adhesion molecule (NCAM) has been noted to be highly associated with 
      SCLC. We examined the ability and efficiency of a streptavidin-Protein A (ST-PA) 
      fusion protein complexed with an anti-NCAM monoclonal antibody (Mab) to transfer 
      biotinylated beta-galactosidase into human SCLC cell lines NCI-H69, NCI-H526, and 
      NCI-H446. When the surface molecule NCAM was targeted with this system, more than 
      99% of the targeted cells internalized and exhibited beta-galactosidase activity. 
      In addition, we evaluated cytotoxic activity against SCLC lines NCI-H69 and 
      NCI-H526 by efficient delivery of biotinylated glucose oxidase using the same 
      ST-PA/anti-NCAM Mab complex. Cytotoxicity of the transduced cells (SCLC) was 
      10-fold and 100-fold greater, respectively, than the glucose oxidase control. 
      This system could be widely applied for specific therapy of cancer cells by 
      targeting unique surface molecules (antigens) using the corresponding Mab/ST-PA 
      complex to transfer a variety of effector molecules; e.g., immunotoxic compounds, 
      into target cells with a high degree of efficiency and specificity.
FAU - Yu, A
AU  - Yu A
AD  - Department of Medicine, New York University School of Medicine, and Chest 
      Service, Bellevue Hospital Center, New York, USA.
FAU - Choi, J
AU  - Choi J
FAU - Ohno, K
AU  - Ohno K
FAU - Levin, B
AU  - Levin B
FAU - Rom, W N
AU  - Rom WN
FAU - Meruelo, D
AU  - Meruelo D
LA  - eng
GR  - CA22247/CA/NCI NIH HHS/United States
GR  - CA68498/CA/NCI NIH HHS/United States
GR  - MOI RR00096/RR/NCRR NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - DNA Cell Biol
JT  - DNA and cell biology
JID - 9004522
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Neural Cell Adhesion Molecules)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Toxins, Biological)
RN  - 6SO6U10H04 (Biotin)
RN  - 9013-20-1 (Streptavidin)
RN  - EC 1.1.3.4 (Glucose Oxidase)
RN  - EC 3.2.1.23 (beta-Galactosidase)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/genetics
MH  - Biotin/genetics
MH  - Carcinoma, Small Cell/*genetics/pathology/therapy
MH  - Cell Count
MH  - Cell Survival
MH  - *Gene Transfer Techniques
MH  - Glucose Oxidase/genetics
MH  - Humans
MH  - Lung Neoplasms/*genetics/pathology/therapy
MH  - Mice
MH  - Mice, SCID
MH  - Neural Cell Adhesion Molecules/genetics/immunology
MH  - Recombinant Fusion Proteins/genetics
MH  - Streptavidin/*genetics
MH  - Toxins, Biological/*genetics
MH  - Transduction, Genetic
MH  - Tumor Cells, Cultured
MH  - beta-Galactosidase/genetics
EDAT- 2000/08/17 11:00
MHDA- 2000/08/19 11:00
CRDT- 2000/08/17 11:00
PHST- 2000/08/17 11:00 [pubmed]
PHST- 2000/08/19 11:00 [medline]
PHST- 2000/08/17 11:00 [entrez]
AID - 10.1089/10445490050085870 [doi]
PST - ppublish
SO  - DNA Cell Biol. 2000 Jul;19(7):383-8. doi: 10.1089/10445490050085870.