PMID- 10945748
OWN - NLM
STAT- MEDLINE
DCOM- 20010118
LR  - 20220311
IS  - 0105-1873 (Print)
IS  - 0105-1873 (Linking)
VI  - 43
IP  - 2
DP  - 2000 Aug
TI  - Activity of human contact allergens in the murine local lymph node assay.
PG  - 95-102
AB  - The murine local lymph node assay (LLNA) is a predictive test for the 
      identification of chemicals that have the potential to cause skin sensitization. 
      Since its original development, the assay has been the subject of national and 
      international evaluation studies and extensive comparisons with guinea pig tests 
      and human data. On the basis of these investigations, the LLNA has recently been 
      endorsed by ICCVAM (Interagency Coordinating Committee on the Validation of 
      Alternative Methods) as a stand-alone method for skin sensitization hazard 
      identification. At the same time, ICCVAM confirmed that, although the LLNA is not 
      an in vitro method, it does represent a refinement in the way animals are used 
      and can provide a means for reducing the number of animals used in sensitization 
      hazard assessment. The investigations described here were designed to explore 
      further the ability of the LLNA to identify accurately those chemicals that cause 
      allergic contact dermatitis in humans. To that end we have measured, amongst 3 
      independent laboratories, LLNA responses induced by a total of 18 test chemicals, 
      11 of which are known to cause skin sensitization and 7 of which are believed not 
      to be associated with any significant evidence of allergic contact dermatitis in 
      humans. The LLNA correctly classified 16 of the 18 materials. The 11 chemicals 
      tested which are associated with allergic contact dermatitis in humans were found 
      to be positive in the LLNA. Of the 7 materials believed to be non-sensitizers, 5 
      were negative in the LLNA and 2 produced positive results. Collectively, these 
      data provide additional evidence that the LLNA is able to discriminate skin 
      sensitizers from those chemicals which do not possess a significant skin 
      sensitization potential and thus provides a method for hazard identification that 
      offers important animal welfare benefits.
FAU - Ryan, C A
AU  - Ryan CA
AD  - The Procter & Gamble Company, Cincinnati, Ohio 45253, USA.
FAU - Gerberick, G F
AU  - Gerberick GF
FAU - Cruse, L W
AU  - Cruse LW
FAU - Basketter, D A
AU  - Basketter DA
FAU - Lea, L
AU  - Lea L
FAU - Blaikie, L
AU  - Blaikie L
FAU - Dearman, R J
AU  - Dearman RJ
FAU - Warbrick, E V
AU  - Warbrick EV
FAU - Kimber, I
AU  - Kimber I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Contact Dermatitis
JT  - Contact dermatitis
JID - 7604950
RN  - 0 (Allergens)
SB  - IM
MH  - Allergens/*adverse effects
MH  - Animals
MH  - Dermatitis, Allergic Contact/*etiology
MH  - Female
MH  - Humans
MH  - *Local Lymph Node Assay
MH  - Mice
MH  - Mice, Inbred CBA
MH  - Predictive Value of Tests
EDAT- 2000/08/17 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/08/17 11:00
PHST- 2000/08/17 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/08/17 11:00 [entrez]
AID - 10.1034/j.1600-0536.2000.043002095.x [doi]
PST - ppublish
SO  - Contact Dermatitis. 2000 Aug;43(2):95-102. doi: 
      10.1034/j.1600-0536.2000.043002095.x.