PMID- 10946247
OWN - NLM
STAT- MEDLINE
DCOM- 20001030
LR  - 20210224
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 469
IP  - 1
DP  - 2000 Aug 21
TI  - Phenolic acids inhibit chloroplast mutagenesis in Euglena gracilis.
PG  - 107-14
AB  - The mutagenicity (bleaching activity) of ofloxacin (43 microM) and acridine 
      orange (AO) (13.5 microM) in Euglena gracilis is inhibited by plant phenolics. 
      Caffeic acid (CA), p-coumaric acid (PCA), ferulic acid (FA) and gentisic acid 
      (GA) (25, 50, 100 and 250 microM) exhibited a significant concentration-dependent 
      inhibitory effect against ofloxacin-induced mutagenicity, which was very 
      effectively eliminated by the highest concentration of all four of those phenolic 
      acids. The mutagenicity of AO was also significantly reduced in the presence of 
      CA, PCA and FA (25, 50, 100 and 250 microM). However, GA exhibited no significant 
      activity, even at the concentration of 250 microM. Based on the UV 
      spectrophotometric measurements, we suggest that the antimutagenic effect of CA, 
      PCA, FA and GA resulted from the scavenging of reactive oxygen species (ROS) 
      produced by ofloxacin. On the other hand, the reduction of AO-induced 
      mutagenicity correlates with the binding capabilities of CA, PCA and FA, with the 
      exception of GA.
FAU - Krizkova, L
AU  - Krizkova L
AD  - Institute of Cell Biology, Faculty of Science, Comenius University, Odborarske 
      nam. 5, 811 07, Bratislava, Slovak Republic. krizkova@m2.fedu.uniba.sk
FAU - Nagy, M
AU  - Nagy M
FAU - Polonyi, J
AU  - Polonyi J
FAU - Dobias, J
AU  - Dobias J
FAU - Belicova, A
AU  - Belicova A
FAU - Grancai, D
AU  - Grancai D
FAU - Krajcovic, J
AU  - Krajcovic J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Antimutagenic Agents)
RN  - 0 (Antioxidants)
RN  - 0 (Caffeic Acids)
RN  - 0 (Coumaric Acids)
RN  - 0 (Gentisates)
RN  - 0 (Hydroxybenzoates)
RN  - 0 (Mutagens)
RN  - 0 (Propionates)
RN  - A4P49JAZ9H (Ofloxacin)
RN  - AVM951ZWST (ferulic acid)
RN  - F30N4O6XVV (Acridine Orange)
RN  - I3P9R8317T (phenolic acid)
RN  - IBS9D1EU3J (p-coumaric acid)
RN  - U2S3A33KVM (caffeic acid)
RN  - VP36V95O3T (2,5-dihydroxybenzoic acid)
SB  - IM
MH  - Acridine Orange/*toxicity
MH  - Animals
MH  - Antimutagenic Agents/*pharmacology
MH  - Antioxidants/pharmacology
MH  - Caffeic Acids/pharmacology
MH  - Chloroplasts/*drug effects/genetics
MH  - Coumaric Acids/pharmacology
MH  - Euglena gracilis/drug effects/*genetics
MH  - *Gentisates
MH  - Hydroxybenzoates/*pharmacology
MH  - Mutagenicity Tests
MH  - Mutagens/*toxicity
MH  - Ofloxacin/antagonists & inhibitors/*toxicity
MH  - Propionates
EDAT- 2000/08/18 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/08/18 11:00
PHST- 2000/08/18 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/08/18 11:00 [entrez]
AID - S1383571800000590 [pii]
AID - 10.1016/s1383-5718(00)00059-0 [doi]
PST - ppublish
SO  - Mutat Res. 2000 Aug 21;469(1):107-14. doi: 10.1016/s1383-5718(00)00059-0.