PMID- 10947148
OWN - NLM
STAT- MEDLINE
DCOM- 20010125
LR  - 20190915
IS  - 1073-2322 (Print)
IS  - 1073-2322 (Linking)
VI  - 14
IP  - 2
DP  - 2000 Aug
TI  - Production of IL-6 and MCP-1 by the human peritoneum in vivo during major 
      abdominal surgery.
PG  - 91-4
AB  - Both inflammatory and anti-inflammatory mediators are released into the 
      circulation during major abdominal surgery. In addition, some of these mediators 
      have been detected postoperatively in peritoneal fluids. Thus, it appears that 
      the peritoneum may be a potential source of circulating immunomodulators 
      following major abdominal surgery. With this in mind, we quantified the 
      intraoperative production of interleukin (IL)-6 and monocyte chemoattractant 
      protein-1 (MCP-1) by human peritoneum. A small chamber was sewed to the parietal 
      peritoneum of 19 patients at the beginning of the operation. This chamber was 
      perfused with buffered salt solution, and the perfusate was collected hourly and 
      assayed for IL-6 and MCP-1 concentrations by enzyme-linked immunosorbent assay. 
      Expression of the corresponding mRNAs was determined by reverse-transcription 
      polymerase chain reaction from additional peritoneal biopsies taken at the 
      beginning and at the end of operation. Peritoneal production of IL-6 and MCP-1 
      started within the first hour of operation and continued with increasing amounts 
      of up to 435 (43-1925) pg/cm2/h [median (range)] of IL-6 and 435 (59-1930) 
      pg/cm2/h of MCP-1. There was induction of peritoneal IL-6 and MCP-1 mRNA 
      expression. A suppressed MCP-1 production was seen only in one patient who 
      suffered from severe septic complications in the postoperative course. Using a 
      new technique that allows for the quantification of local cytokine production in 
      vivo, we demonstrated that the peritoneum rapidly reacts to abdominal surgery 
      with increased production of IL-6 and MCP-1. Early detection of impaired 
      production may help to identify patients at risk of postoperative septic 
      complications.
FAU - Riese, J
AU  - Riese J
AD  - Department of Surgery, University of Erlangen-Nuremberg, Erlangen, Germany.
FAU - Schoolmann, S
AU  - Schoolmann S
FAU - Beyer, A
AU  - Beyer A
FAU - Denzel, C
AU  - Denzel C
FAU - Hohenberger, W
AU  - Hohenberger W
FAU - Haupt, W
AU  - Haupt W
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Shock
JT  - Shock (Augusta, Ga.)
JID - 9421564
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-6)
SB  - IM
MH  - Abdomen/*surgery
MH  - Adult
MH  - Aged
MH  - Chemokine CCL2/*biosynthesis/genetics
MH  - Disease Susceptibility
MH  - Female
MH  - Gastrointestinal Neoplasms/surgery
MH  - Gene Expression Regulation
MH  - Humans
MH  - Immunocompromised Host
MH  - Interleukin-6/*biosynthesis/genetics
MH  - Intraoperative Period
MH  - Male
MH  - Middle Aged
MH  - Pancreatic Neoplasms/surgery
MH  - Peritoneum/*metabolism
MH  - Postoperative Complications/epidemiology/*immunology
MH  - Prostheses and Implants
MH  - Risk Factors
MH  - Sepsis/epidemiology/*immunology
EDAT- 2000/08/18 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/08/18 11:00
PHST- 2000/08/18 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/08/18 11:00 [entrez]
AID - 10.1097/00024382-200014020-00002 [doi]
PST - ppublish
SO  - Shock. 2000 Aug;14(2):91-4. doi: 10.1097/00024382-200014020-00002.