PMID- 10947818
OWN - NLM
STAT- MEDLINE
DCOM- 20000919
LR  - 20190815
IS  - 0953-816X (Print)
IS  - 0953-816X (Linking)
VI  - 12
IP  - 7
DP  - 2000 Jul
TI  - Dedifferentiation of intrinsic response properties of motoneurons in organotypic 
      cultures of the spinal cord of the adult turtle.
PG  - 2397-404
AB  - Explant cultures from the spinal cord of adult turtles were established and used 
      to study the sensitivity of the intrinsic response properties of motoneurons to 
      the changes in connectivity and milieu imposed by isolation in culture. 
      Transverse sections 700 microm thick were explanted on cover slips and maintained 
      in roller-tube cultures in medium containing serum and the growth factors 
      brain-derived neurotrophin factor (BDNF), neurotrophin-3 (NT3), glial cell 
      line-derived neurotrophic factor (GDNF) and ciliary neurotrophic factor (CNTF). 
      The gross morphology of acute sections was maintained after 4 weeks in culture. 
      Cell bodies of motoneurons remained stainable in fixed cultures with an antibody 
      against choline acetyltransferase (ChAT) throughout the culture period. During 
      culture, motoneurons maintained stable resting membrane potentials and were 
      contacted by functional synapses. The ability to generate action potentials was 
      also preserved as was delayed inward rectification and generation of calcium 
      spikes in the presence of tetra-ethyl ammonium (TEA). In response to 
      depolarization, however, motoneurons presented strong outward rectification, and 
      only 41% of the cells recorded from maintained the ability to fire repetitively. 
      By the second week in culture, a fraction of motoneurons displayed fast and slow 
      transient outward rectification and low-threshold calcium spikes, features not 
      seen in turtle motoneurons in acute slices. On the other hand, properties 
      mediated by L-type Ca2+ channels disappeared during the first few days in 
      culture. Our observations show that the phenotypical intrinsic response 
      properties of mature spinal motoneurons are modified in explant cultures. The 
      properties acquired resemble the properties in juvenile motoneurons in several 
      species of terrestrial vertebrates.
FAU - Perrier, J F
AU  - Perrier JF
AD  - Department of Medical Physiology, Panum Institute, University of Copenhagen, 
      Denmark.
FAU - Noraberg, J
AU  - Noraberg J
FAU - Simon, M
AU  - Simon M
FAU - Hounsgaard, J
AU  - Hounsgaard J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Chlorides)
RN  - 0 (Ciliary Neurotrophic Factor)
RN  - 0 (Glial Cell Line-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (Neurotrophin 3)
RN  - 0TQU7668EI (Cycloleucine)
RN  - 111900-32-4 (1-amino-1,3-dicarboxycyclopentane)
RN  - 1KSV9V4Y4I (Cesium)
RN  - 4368-28-9 (Tetrodotoxin)
RN  - 66-40-0 (Tetraethylammonium)
RN  - GNR9HML8BA (cesium chloride)
SB  - IM
MH  - Action Potentials/drug effects/physiology
MH  - Age Factors
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/pharmacology
MH  - Cell Differentiation/drug effects/physiology
MH  - Cesium/pharmacology
MH  - Chlorides/pharmacology
MH  - Ciliary Neurotrophic Factor/pharmacology
MH  - Cycloleucine/analogs & derivatives/pharmacology
MH  - Electrophysiology
MH  - Glial Cell Line-Derived Neurotrophic Factor
MH  - Motor Neurons/*cytology/*physiology
MH  - *Nerve Growth Factors
MH  - Nerve Tissue Proteins/pharmacology
MH  - Neuroprotective Agents/pharmacology
MH  - Neurotrophin 3/pharmacology
MH  - Organ Culture Techniques
MH  - Spinal Cord/*cytology/*physiology
MH  - Synapses/physiology
MH  - Tetraethylammonium/pharmacology
MH  - Tetrodotoxin/pharmacology
MH  - Turtles
EDAT- 2000/08/18 11:00
MHDA- 2000/09/23 11:01
CRDT- 2000/08/18 11:00
PHST- 2000/08/18 11:00 [pubmed]
PHST- 2000/09/23 11:01 [medline]
PHST- 2000/08/18 11:00 [entrez]
AID - ejn134 [pii]
AID - 10.1046/j.1460-9568.2000.00134.x [doi]
PST - ppublish
SO  - Eur J Neurosci. 2000 Jul;12(7):2397-404. doi: 10.1046/j.1460-9568.2000.00134.x.