PMID- 10951366
OWN - NLM
STAT- MEDLINE
DCOM- 20000919
LR  - 20190910
IS  - 0021-9304 (Print)
IS  - 0021-9304 (Linking)
VI  - 52
IP  - 2
DP  - 2000 Nov
TI  - Characterization of osteoblast-like behavior of cultured bone marrow stromal 
      cells on various polymer surfaces.
PG  - 279-84
AB  - The creation of novel bone substitutes requires a detailed understanding of the 
      interaction between cells and materials. This study was designed to test certain 
      polymers, specifically poly(caprolactone) (PCL), poly(D,L-lactic-CO-glycolic 
      acid) (PLGA), and combinations of these polymers for their ability to support 
      bone marrow stromal cell proliferation and differentiation. Bone marrow stromal 
      cells were cultured from New Zealand White rabbits and were seeded onto glass 
      slides coated with a thin layer of PCL, PLGA, and combinations of these two 
      polymers in both a 40:60 and a 10:90 ratio. Growth curves were compared. At the 
      end of 2 weeks, the cells were stained for both matrix mineralization and 
      alkaline phosphatase activity. There was no statistically significant difference 
      in growth rate of the cells on any polymer or polymer combination. However, there 
      was a striking difference in Von Kossa staining and alkaline phosphatase 
      staining. Cells on PCL did not show Von Kossa staining or alkaline phosphatase 
      staining. However, in the 40:60 and 10:90 blends, there was both positive Von 
      Kossa and alkaline phosphatase staining. These data indicate that PCL alone may 
      not be a satisfactory material for the creation of a bone substitute. However, it 
      may be used in combination with PLGA for the creation of a bone substitute 
      material.
CI  - Copyright 2000 John Wiley & Sons, Inc.
FAU - Calvert, J W
AU  - Calvert JW
AD  - Division of Plastic and Reconstructive Surgery, University of Pittsburgh Medical 
      Center, 6B Scaife Hall, Pittsburgh, Pennsylvania, USA. calvertjw@msx.upmc.edu
FAU - Marra, K G
AU  - Marra KG
FAU - Cook, L
AU  - Cook L
FAU - Kumta, P N
AU  - Kumta PN
FAU - DiMilla, P A
AU  - DiMilla PA
FAU - Weiss, L E
AU  - Weiss LE
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Biomed Mater Res
JT  - Journal of biomedical materials research
JID - 0112726
RN  - 0 (Biocompatible Materials)
RN  - 0 (Polymers)
SB  - IM
MH  - Animals
MH  - *Biocompatible Materials
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Osteoblasts/*cytology
MH  - *Polymers
MH  - Rabbits
MH  - Stromal Cells/*cytology
EDAT- 2000/08/22 11:00
MHDA- 2000/09/23 11:01
CRDT- 2000/08/22 11:00
PHST- 2000/08/22 11:00 [pubmed]
PHST- 2000/09/23 11:01 [medline]
PHST- 2000/08/22 11:00 [entrez]
AID - 10.1002/1097-4636(200011)52:2<279::AID-JBM6>3.0.CO;2-8 [pii]
AID - 10.1002/1097-4636(200011)52:2<279::aid-jbm6>3.0.co;2-8 [doi]
PST - ppublish
SO  - J Biomed Mater Res. 2000 Nov;52(2):279-84. doi: 
      10.1002/1097-4636(200011)52:2<279::aid-jbm6>3.0.co;2-8.