PMID- 10951399
OWN - NLM
STAT- MEDLINE
DCOM- 20001024
LR  - 20071115
IS  - 0022-3417 (Print)
IS  - 0022-3417 (Linking)
VI  - 192
IP  - 1
DP  - 2000 Sep
TI  - Differentiation of multicentric origin from intra-organ metastatic spread of 
      hepatocellular carcinomas by comparative genomic hybridization.
PG  - 43-51
AB  - In hepatocellular carcinoma (HCC), multifocal growth may be due to intrahepatic 
      metastatic spread or to the multicentric origin of clonal neoplasms. Although 
      this issue is of potential clinical and prognostic importance, reliable 
      differentiation cannot be achieved using clinical or morphological criteria 
      alone. In this study, comparative genomic hybridization (CGH) was used to 
      differentiate between metastatic spread and multicentric growth in two cases of 
      HCC. In the first case, six carcinoma nodules were examined. The affected 
      chromosomes and their pattern of aberrations were almost identical for all six 
      nodules. In addition to aberrations of chromosomes 1, 4, 9, and 13, further 
      aberrations were observed for chromosomes 2, 5, 7, and 17, which are less typical 
      for HCC. These findings were seen as indicative of metastatic spread of the HCC. 
      In the second case, 75% (3/4) of the nodules showed comparable aberration 
      patterns involving chromosomes 1, 4, 8, 13, and 17, together with a number of 
      further aberrations also not frequently seen in HCC including chromosomes 5, 7, 
      10, 12, 14, and 18. Chromosomes 4, 5, 8, 10, and 12 were also altered in the 
      fourth nodule examined for this case, but they exhibited a unique aberration 
      pattern. Additionally, gain of chromosome 15q was seen in only this fourth 
      nodule. In the two cases examined, metastatic spread and multicentric origin of 
      HCC could be differentiated by different patterns of karyotypic change. The CGH 
      results were confirmed by fluorescence in situ hybridization (FISH). In 
      conclusion, CGH facilitates the differentiation of multicentric growth from 
      metastatic spread in HCC and appears to be superior to techniques previously used 
      to resolve this clinically important diagnostic problem.
CI  - Copyright 2000 John Wiley & Sons, Ltd.
FAU - Wilkens, L
AU  - Wilkens L
AD  - Institute of Pathology, Hannover Medical School, Germany.
FAU - Bredt, M
AU  - Bredt M
FAU - Flemming, P
AU  - Flemming P
FAU - Klempnauer, J
AU  - Klempnauer J
FAU - Heinrich Kreipe, H
AU  - Heinrich Kreipe H
LA  - eng
PT  - Case Reports
PT  - Journal Article
PL  - England
TA  - J Pathol
JT  - The Journal of pathology
JID - 0204634
SB  - IM
MH  - Adult
MH  - Aged
MH  - Carcinoma, Hepatocellular/*genetics/pathology/*secondary
MH  - *Chromosome Aberrations
MH  - Diagnosis, Differential
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Liver Neoplasms/*genetics/pathology
MH  - Male
MH  - Neoplastic Stem Cells/pathology
MH  - Nucleic Acid Hybridization
EDAT- 2000/08/22 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/08/22 11:00
PHST- 2000/08/22 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/08/22 11:00 [entrez]
AID - 10.1002/1096-9896(2000)9999:9999<::AID-PATH671>3.0.CO;2-Z [pii]
AID - 10.1002/1096-9896(2000)9999:9999<::AID-PATH671>3.0.CO;2-Z [doi]
PST - ppublish
SO  - J Pathol. 2000 Sep;192(1):43-51. doi: 
      10.1002/1096-9896(2000)9999:9999<::AID-PATH671>3.0.CO;2-Z.