PMID- 10951502
OWN - NLM
STAT- MEDLINE
DCOM- 20001002
LR  - 20061115
IS  - 1526-954X (Print)
IS  - 1526-954X (Linking)
VI  - 27
IP  - 3
DP  - 2000 Jul
TI  - Rescue of embryonic lethality in hepatocyte growth factor/scatter factor knockout 
      mice.
PG  - 99-103
AB  - While the targeted disruption of a gene is a powerful tool for investigating the 
      physiological functions of that gene, disruption of a gene essential for 
      embryogenesis leads to embryonic death. Rescue of the defect(s) causing embryonic 
      death should promote survival, thus permitting further evaluation of the roles 
      that the gene plays later in the developmental process. Disruption of the gene 
      for mouse hepatocyte growth factor/scatter factor (HGF/SF) leads to middle-stage 
      embryonic lethality because of a defect in placental development. Here we report 
      that a single injection of HGF/SF at embryonic day 9.5 (E9.5) into the amniotic 
      cavity of HGF/SF(-/-) embryos rescued the placental defect and resulted in the 
      survival of the embryos until term. Histological analysis suggested that HGF/SF 
      is also required at the late stage of development for tissue organogenesis. Thus, 
      injection of a secreted factor can be a useful method to rescue the defects 
      causing embryonic lethality.
FAU - Uehara, Y
AU  - Uehara Y
FAU - Mori, C
AU  - Mori C
FAU - Noda, T
AU  - Noda T
FAU - Shiota, K
AU  - Shiota K
FAU - Kitamura, N
AU  - Kitamura N
LA  - eng
PT  - Letter
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genesis
JT  - Genesis (New York, N.Y. : 2000)
JID - 100931242
RN  - 67256-21-7 (Hepatocyte Growth Factor)
SB  - IM
MH  - Amniotic Fluid
MH  - Animals
MH  - Embryonic and Fetal Development/*physiology
MH  - Fetal Death/*prevention & control
MH  - Gestational Age
MH  - Hepatocyte Growth Factor/deficiency/genetics/*pharmacology/*physiology
MH  - Liver/drug effects/embryology
MH  - Mice
MH  - Mice, Knockout
MH  - Muscle, Skeletal/drug effects/embryology
MH  - Placenta/drug effects/*physiology
EDAT- 2000/08/22 11:00
MHDA- 2000/10/07 11:01
CRDT- 2000/08/22 11:00
PHST- 2000/08/22 11:00 [pubmed]
PHST- 2000/10/07 11:01 [medline]
PHST- 2000/08/22 11:00 [entrez]
AID - 10.1002/1526-968X(200007)27:3<99::AID-GENE20>3.0.CO;2-Y [pii]
PST - ppublish
SO  - Genesis. 2000 Jul;27(3):99-103.