PMID- 10952415
OWN - NLM
STAT- MEDLINE
DCOM- 20001207
LR  - 20061115
IS  - 0272-457X (Print)
IS  - 0272-457X (Linking)
VI  - 19
IP  - 3
DP  - 2000 Jun
TI  - Production, characterization, and uses of monoclonal antibodies against porcine 
      reproductive and respiratory syndrome virus 3' untranslated region and 
      nucleoprotein RNA binding proteins.
PG  - 263-7
AB  - Previous studies in our laboratory of interactions between the 3' untranslated 
      region of porcine reproductive and respiratory syndrome virus (PRRSV) and MARC 
      145 cell cytoplasmic proteins have identified 11 RNA binding proteins. Here, we 
      report the production and characterization of monoclonal antibodies (MAbs) 
      against a 67-kD RNA binding protein of MARC 145 tissue cultured cells. Of the 
      MAbs produced, 11 were reactive in ELISA with 67-kD protein. Immunoprecipitation 
      tests showed that six clones precipitated a protein of 67 kD, and one clone 
      recognized a multiple protein bands of 45, 37, and 27 kD. Western blotting showed 
      that these clones detected two proteins of 67 and 55 kD. Indirect fluorescent 
      antibody staining of testing of PRRSV-infected cells with these MAbs revealed 
      diffuse cytoplasmic staining and intense perinuclear staining to one side of the 
      nucleus. The presence of the double membrane vesicles in the same region in 
      PRRSV-infected alveolar macrophages suggests that these RNA binding proteins 
      might have a role in their formation.
FAU - Shanmukhappa, K
AU  - Shanmukhappa K
AD  - Department of Diagnostic Medicine-Pathobiology, College of Veterinary Medicine, 
      Kansas State University, Manhattan 66506, USA.
FAU - Majhdi, F
AU  - Majhdi F
FAU - Kapil, S
AU  - Kapil S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Hybridoma
JT  - Hybridoma
JID - 8202424
RN  - 0 (3' Untranslated Regions)
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Nucleoproteins)
RN  - 0 (RNA-Binding Proteins)
SB  - IM
MH  - 3' Untranslated Regions/*genetics/*immunology/metabolism
MH  - Animals
MH  - Antibodies, Monoclonal/*chemistry/*metabolism
MH  - Blotting, Western
MH  - Cell Line
MH  - Hybridomas
MH  - Nucleoproteins/*immunology
MH  - Porcine respiratory and reproductive syndrome 
      virus/*genetics/*immunology/metabolism
MH  - Precipitin Tests
MH  - RNA-Binding Proteins/*immunology
MH  - Swine
EDAT- 2000/08/22 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/08/22 11:00
PHST- 2000/08/22 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/08/22 11:00 [entrez]
AID - 10.1089/02724570050109666 [doi]
PST - ppublish
SO  - Hybridoma. 2000 Jun;19(3):263-7. doi: 10.1089/02724570050109666.