PMID- 10953311
OWN - NLM
STAT- MEDLINE
DCOM- 20000831
LR  - 20041117
IS  - 0250-7005 (Print)
IS  - 0250-7005 (Linking)
VI  - 20
IP  - 4
DP  - 2000 Jul-Aug
TI  - Relationship between binuclear and multinuclear cells in giant cell tumor of 
      bone.
PG  - 2463-7
AB  - Giant cell tumor of bone (GCT) consists of stromal and multinuclear type tumor 
      cells. Although most people believe that the stromal cells are mononuclear, we 
      recently found the existence of many binuclear cells among stromal cells using 
      DNA cytofluorometric examination. This study, using 18 tumors of GCT was 
      conducted to elucidate the cell biological significance of the binuclear cell, 
      especially its relationship to multinuclear cell formation or tumor cell 
      proliferation. The investigation was carried out by means of DNA-RNA 
      cytofluorometry with acridine orange (AO) and histological method. Using 
      fluorescence microscopic observation, we counted the numbers of both mononuclear 
      and binuclear cells and calculated the index of % BNC, which expresses the 
      frequency (percentage) of binuclear cells in a population of mononuclear and 
      binuclear cells. The index of % S-G2 obtained by DNA-RNA cytofluorometry showed 
      the frequency (percentage) of mononuclear cells in the S and G2 phases of the 
      cell cycle. In the histological study, we counted the numbers of multinuclear 
      giant cells with more than 3 nuclei in the cytoplasm and stromal cells including 
      mononuclear and binuclear cells and calculated MNS/SC, which showed the 
      percentage of multinuclear cells in the stromal cells in the microscopic field. 
      Eight tumors showed a value of % BNC greater than 10% and 2 had a value of 40%. 
      The index of % BNC significantly correlated with the average value of MNC/SC in 
      all tumors. There was no significant correlation between % BNC and the average 
      value of % S-G2, in 18 tumors although 4 tumors having a % BNC value greater than 
      20% showed a % S-G2 value greater than 12% in 18 tumors. These results revealed 
      the presence of many binuclear cells among stromal cells of GCT and suggested 
      that these binuclear cells might be formed in association with the active 
      proliferation of mononuclear cells and closely relate to the formation of 
      multinuclear giant cells.
FAU - Kusuzaki, K
AU  - Kusuzaki K
AD  - Department of Orthopaedic Surgery, Kyoto Prefectural University of Medicine, 
      Japan. kusu@kamogawa.pathol.kpu-m.ac.jp
FAU - Takeshita, H
AU  - Takeshita H
FAU - Murata, H
AU  - Murata H
FAU - Hashiguchi, S
AU  - Hashiguchi S
FAU - Nozaki, T
AU  - Nozaki T
FAU - Emoto, K
AU  - Emoto K
FAU - Ashihara, T
AU  - Ashihara T
FAU - Hirasawa, Y
AU  - Hirasawa Y
LA  - eng
PT  - Journal Article
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
RN  - 0 (DNA, Neoplasm)
RN  - 0 (RNA, Neoplasm)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Bone Neoplasms/*pathology
MH  - Cell Cycle
MH  - Cell Division
MH  - DNA, Neoplasm/analysis
MH  - Female
MH  - Giant Cell Tumor of Bone/*pathology
MH  - Humans
MH  - Male
MH  - RNA, Neoplasm/analysis
EDAT- 2000/08/23 11:00
MHDA- 2000/09/02 11:01
CRDT- 2000/08/23 11:00
PHST- 2000/08/23 11:00 [pubmed]
PHST- 2000/09/02 11:01 [medline]
PHST- 2000/08/23 11:00 [entrez]
PST - ppublish
SO  - Anticancer Res. 2000 Jul-Aug;20(4):2463-7.