PMID- 10954573
OWN - NLM
STAT- MEDLINE
DCOM- 20000927
LR  - 20231213
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 74
IP  - 18
DP  - 2000 Sep
TI  - The valine anticodon and valylatability of Peanut clump virus RNAs are not 
      essential but provide a modest competitive advantage in plants.
PG  - 8720-5
AB  - The role of valine aminoacylation of the two genomic RNAs of Peanut clump virus 
      (PCV) was studied by comparing the amplification in vivo of RNAs with GAC, 
      GDeltaC, or CCA anticodons in the tRNA-like structure (TLS) present at the 3' end 
      of each viral RNA. The PCV RNA1 TLS of isolate PCV2 possesses a GAC anticodon and 
      is capable of highly efficient valylation, whereas the RNA2 TLS has a GDeltaC 
      anticodon that does not support valylation. The presence in RNA1 of GDeltaC or 
      CCA anticodons that conferred nonvalylatability resulted in about 2- to 4-fold 
      and a 14- to 24-fold reduction, respectively, in RNA accumulations in tobacco 
      BY-2 protoplasts inoculated with the RNA1 variants together with wild-type 
      RNA2(GDeltaC). No differences in RNA levels were observed among protoplasts 
      inoculated with the three variant RNA2s in the presence of wild-type RNA1(GAC). 
      All combinations of valylatable and nonvalylatable RNAs 1 and 2 were similarly 
      infectious in Nicotiana benthamiana plants, and viral RNAs accumulated to similar 
      levels; all input TLS sequences were present unchanged in apical leaves. In 
      direct competition experiments in N. benthamiana plants, however, both RNA1 and 
      RNA2 with GAC valylatable anticodons outcompeted the nonvalylatable variants. We 
      conclude that valylation provides a small but significant replicational advantage 
      to both PCV RNAs. Sequence analysis of the TLS from RNA2 of a second PCV isolate, 
      PO2A, revealed the presence of an intact GAC valine anticodon, suggesting that 
      the differential valylation of the genomic RNAs of isolate PCV2 is not a general 
      characteristic of PCV.
FAU - Matsuda, D
AU  - Matsuda D
AD  - Department of Microbiology, Oregon State University, Corvallis, Oregon 
      97331-3804, USA.
FAU - Dunoyer, P
AU  - Dunoyer P
FAU - Hemmer, O
AU  - Hemmer O
FAU - Fritsch, C
AU  - Fritsch C
FAU - Dreher, T W
AU  - Dreher TW
LA  - eng
GR  - GM-54610/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Anticodon)
RN  - 0 (RNA, Transfer, Amino Acyl)
RN  - 0 (RNA, Transfer, Val)
RN  - 0 (RNA, Viral)
RN  - HG18B9YRS7 (Valine)
SB  - IM
MH  - Anticodon
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Molecular Sequence Data
MH  - Mutagenesis, Site-Directed
MH  - Mutation
MH  - *Plants, Toxic
MH  - Protoplasts/virology
MH  - RNA Viruses/chemistry/*genetics/metabolism
MH  - RNA, Transfer, Amino Acyl/chemistry/genetics/metabolism
MH  - RNA, Transfer, Val/chemistry/genetics/metabolism
MH  - RNA, Viral/chemistry/*genetics/metabolism
MH  - Sequence Analysis, RNA
MH  - Nicotiana/*virology
MH  - Valine/*genetics
PMC - PMC116383
EDAT- 2000/08/23 11:00
MHDA- 2000/09/30 11:01
PMCR- 2000/09/01
CRDT- 2000/08/23 11:00
PHST- 2000/08/23 11:00 [pubmed]
PHST- 2000/09/30 11:01 [medline]
PHST- 2000/08/23 11:00 [entrez]
PHST- 2000/09/01 00:00 [pmc-release]
AID - 0811 [pii]
AID - 10.1128/jvi.74.18.8720-8725.2000 [doi]
PST - ppublish
SO  - J Virol. 2000 Sep;74(18):8720-5. doi: 10.1128/jvi.74.18.8720-8725.2000.