PMID- 10955247
OWN - NLM
STAT- MEDLINE
DCOM- 20010111
LR  - 20190515
IS  - 1058-0468 (Print)
IS  - 1573-7330 (Electronic)
IS  - 1058-0468 (Linking)
VI  - 17
IP  - 4
DP  - 2000 Apr
TI  - Relationships between concentrations of tumor necrosis factor-alpha and nitric 
      oxide in follicular fluid and oocyte quality.
PG  - 222-8
AB  - PURPOSE: Our objective was to explain a relationship between concentrations of 
      tumor necrosis factor-alpha (TNF-alpha) and nitric oxide (NO) in follicular 
      fluid, oocyte quality, and outcomes of in vitro fertilization--embryo transfer 
      (IVF-ET). METHODS: The concentrations of TNF-alpha and NO were measured in 115 
      follicular fluid samples collected from 43 patients undergoing IVF-ET program, 
      due to tubal obstruction, some with endometriosis (8 patients) or hydrosalpinx (5 
      patients). A correlation of these factors concentrations and the oocyte quality, 
      the oocyte maturity, and infertility-associated diseases was analyzed. RESULTS: 
      No correlation was found between concentrations of NO and TNF-alpha in follicular 
      fluid. NO concentrations in follicular fluids were significantly higher in 
      patients with endometriosis (P < 0.001) or hydrosalpinx (P < 0.01) compared to 
      the patients with just tubal obstruction. Follicular NO concentration differences 
      according to oocyte maturity and oocyte quality were not found. In contrast, 
      TNF-alpha concentrations in follicular fluids were significantly higher in poor 
      quality oocytes (P < 0.05) but were not associated with infertility-associated 
      diseases, such as hydrosalphinx or endometriosis,and the oocyte maturity. No 
      significant differences in follicular levels of NO and TNF-alpha as well as 
      IVF-ET parameters of pregnant and nonpregnant groups were revealed. CONCLUSIONS: 
      There is no significant correlation between the concentrations of NO and 
      TNF-alpha in follicular fluid. NO levels in follicular fluid are altered in 
      infertility-associated diseases. However, TNF-alpha levels but not NO levels 
      influence oocyte quality. These results suggest that the production of NO and 
      TNF-alpha in follicular fluid may be regulated via different pathways and can be 
      tempered with infertility-associated diseases, thereby influencing oocyte quality 
      locally.
FAU - Lee, K S
AU  - Lee KS
AD  - Department of Obstetrics and Gynecology, Pusan National University School of 
      Medicine, Korea.
FAU - Joo, B S
AU  - Joo BS
FAU - Na, Y J
AU  - Na YJ
FAU - Yoon, M S
AU  - Yoon MS
FAU - Choi, O H
AU  - Choi OH
FAU - Kim, W W
AU  - Kim WW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Assist Reprod Genet
JT  - Journal of assisted reproduction and genetics
JID - 9206495
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 31C4KY9ESH (Nitric Oxide)
SB  - IM
MH  - Adult
MH  - Embryo Transfer
MH  - Fallopian Tubes/physiology
MH  - Female
MH  - Fertilization in Vitro
MH  - Follicular Fluid/chemistry/*physiology
MH  - Humans
MH  - Nitric Oxide/analysis/*physiology
MH  - *Oocytes/cytology/physiology
MH  - Ovary/physiology
MH  - Ovulation Induction
MH  - Pregnancy
MH  - Pregnancy Rate
MH  - Tumor Necrosis Factor-alpha/analysis/*physiology
PMC - PMC3455467
EDAT- 2000/08/24 11:00
MHDA- 2001/02/28 10:01
PMCR- 2001/04/01
CRDT- 2000/08/24 11:00
PHST- 2000/08/24 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/08/24 11:00 [entrez]
PHST- 2001/04/01 00:00 [pmc-release]
AID - 223480 [pii]
AID - 10.1023/a:1009495913119 [doi]
PST - ppublish
SO  - J Assist Reprod Genet. 2000 Apr;17(4):222-8. doi: 10.1023/a:1009495913119.