PMID- 10956434
OWN - NLM
STAT- MEDLINE
DCOM- 20000928
LR  - 20190915
IS  - 0033-7587 (Print)
IS  - 0033-7587 (Linking)
VI  - 154
IP  - 3
DP  - 2000 Sep
TI  - Radiation-induced translocations in mice: persistence, chromosome specificity, 
      and influence of genetic background.
PG  - 283-92
AB  - The translocation frequency response in the chromosomes of peripheral blood 
      lymphocytes is widely used for radiation biomonitoring and dose estimation. 
      However, this assay is based upon several assumptions that have not been 
      rigorously tested. It is typically assumed that the translocation frequency in 
      blood lymphocytes reflects the level of genomic damage in other hemopoietic 
      tissues and is independent of the chromosome probe and genetic background. We 
      conducted studies to evaluate these assumptions using mice with different genetic 
      backgrounds. Six different whole-chromosome fluorescence in situ hybridization 
      (FISH) probes were used to detect translocations in peripheral blood lymphocytes 
      at multiple times after whole-body irradiation. Translocation frequencies were 
      chromosome-independent at 6 and 16 weeks after exposure but were 
      chromosome-dependent at 1. 5 years after exposure. Similar translocation 
      frequencies were observed in blood, bone marrow and spleen at 1.5 years, 
      supporting previous suggestions that genetically aberrant peripheral blood 
      lymphocytes may derive from precursor populations in hemopoietic tissues. 
      Translocations measured 66 h after irradiation differed among some strains. We 
      conclude that the translocation frequency response is a complex phenotype that is 
      influenced not only by exposure dose but also by genetic background, the choice 
      of chromosome analyzed, and time after exposure. These results raise important 
      considerations for the use of the FISH-based translocation frequency response for 
      radiation dosimetry and biomonitoring.
FAU - Giver, C R
AU  - Giver CR
AD  - Cancer Center, University of California, San Francisco, California 94143, USA.
FAU - Moore, D H 2nd
AU  - Moore DH 2nd
FAU - Pallavicini, M G
AU  - Pallavicini MG
LA  - eng
GR  - CA55356/CA/NCI NIH HHS/United States
GR  - T32CA09215/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Radiat Res
JT  - Radiation research
JID - 0401245
SB  - IM
MH  - Animals
MH  - Biological Assay
MH  - Blood Cells/radiation effects/ultrastructure
MH  - Chromosomes/*radiation effects/ultrastructure
MH  - Female
MH  - Genes, bcl-2
MH  - Genetic Predisposition to Disease
MH  - Hematopoietic System/radiation effects/ultrastructure
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphocytes/*radiation effects/ultrastructure
MH  - Male
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Radiation Injuries, Experimental/*genetics
MH  - Radiation Tolerance/genetics
MH  - Radiometry
MH  - Research Design
MH  - Translocation, Genetic/*radiation effects
EDAT- 2000/08/24 11:00
MHDA- 2000/09/30 11:01
CRDT- 2000/08/24 11:00
PHST- 2000/08/24 11:00 [pubmed]
PHST- 2000/09/30 11:01 [medline]
PHST- 2000/08/24 11:00 [entrez]
AID - 10.1667/0033-7587(2000)154[0283:ritimp]2.0.co;2 [doi]
PST - ppublish
SO  - Radiat Res. 2000 Sep;154(3):283-92. doi: 
      10.1667/0033-7587(2000)154[0283:ritimp]2.0.co;2.