PMID- 10958887
OWN - NLM
STAT- MEDLINE
DCOM- 20001128
LR  - 20221207
IS  - 0014-2999 (Print)
IS  - 0014-2999 (Linking)
VI  - 402
IP  - 3
DP  - 2000 Aug 25
TI  - 3,4-methylenedioxymethamphetamine (MDMA, ecstasy)-induced egr-1 mRNA in rat 
      brain: pharmacological manipulation.
PG  - 215-22
AB  - Using in situ hybridization and immunohistochemical techniques, we examined the 
      expression pattern of egr-1 mRNA and Egr-1 protein in several brain regions 
      following administration of 3, 4-methylenedioxymethamphetamine (MDMA). 
      Furthermore, we also studied the role of N-methyl-D-aspartate (NMDA) receptor, 
      dopamine D(1) receptor, 5-hydroxytryptamine (5-HT) transporter or 5-HT(2A) 
      receptor in the induction of egr-1 mRNA by MDMA. Basal constitutive levels of 
      egr-1 mRNA were detected in control rat brains. A single administration of MDMA 
      (10 mg/kg) caused marked induction of egr-1 mRNA in the prefrontal cortex, 
      striatum and hippocampal dentate gyrus. However, no changes in the egr-1 mRNA 
      levels were detected in the CA1 region of hippocampus and occipital cortex after 
      administration of MDMA (10 mg/kg). Furthermore, the expression of egr-1 mRNA in 
      the prefrontal cortex, striatum and hippocampal dentate gyrus after 
      administration of MDMA (10 mg/kg) was blocked significantly by pretreatment with 
      NMDA receptor antagonist (5R, 
      10S)-(+)-5-methyl-10,11-dihydro-5H-dibenzo[a,b]-cyclohepten-5, 10-imine 
      ((+)-MK801; 1 mg/kg), dopamine D(1) receptor antagonist SCH 23390 (1 mg/kg) or 
      5-HT uptake inhibitor paroxetine (5 mg/kg), but not by 5-HT(2A) receptor 
      antagonist SR46349B (5 mg/kg). However, high basal levels of Egr-1 
      immunoreactivity in the rat brain were not altered by administration of MDMA (10 
      mg/kg). These results suggest that MDMA alters the expression of egr-1 mRNA in 
      several regions of rat brain, and that the expression of egr-1 mRNA by MDMA in 
      the prefrontal cortex, striatum and hippocampal dentate gyrus appears to be 
      mediated, at least in part, by NMDA receptor, dopamine D(1) receptor and 5-HT 
      transporter.
FAU - Shirayama, Y
AU  - Shirayama Y
AD  - Division of Cortical Function Disorders, National Institute of Neuroscience, 
      National Center of Neurology and Psychiatry (NCNP), Tokyo, Kodaira, Japan. 
      yukihiko.shirayama@yale.edu
FAU - Hashimoto, K
AU  - Hashimoto K
FAU - Iyo, M
AU  - Iyo M
FAU - Watanabe, K
AU  - Watanabe K
FAU - Higuchi, T
AU  - Higuchi T
FAU - Minabe, Y
AU  - Minabe Y
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Eur J Pharmacol
JT  - European journal of pharmacology
JID - 1254354
RN  - 0 (Benzazepines)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Dopamine Antagonists)
RN  - 0 (Early Growth Response Protein 1)
RN  - 0 (Egr1 protein, rat)
RN  - 0 (Excitatory Amino Acid Antagonists)
RN  - 0 (Fluorobenzenes)
RN  - 0 (Hallucinogens)
RN  - 0 (Immediate-Early Proteins)
RN  - 0 (Phenols)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Dopamine D1)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 0 (Serotonin Antagonists)
RN  - 0 (Serotonin Uptake Inhibitors)
RN  - 0 (Transcription Factors)
RN  - 130580-02-8 (SR 46349B)
RN  - 41VRH5220H (Paroxetine)
RN  - 6LR8C1B66Q (Dizocilpine Maleate)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
SB  - IM
MH  - Animals
MH  - Benzazepines/pharmacology
MH  - Brain Chemistry/*drug effects
MH  - DNA-Binding Proteins/*biosynthesis
MH  - Dizocilpine Maleate/pharmacology
MH  - Dopamine Antagonists/pharmacology
MH  - Early Growth Response Protein 1
MH  - Excitatory Amino Acid Antagonists/pharmacology
MH  - Fluorobenzenes/pharmacology
MH  - Hallucinogens/*pharmacology
MH  - *Immediate-Early Proteins
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Male
MH  - N-Methyl-3,4-methylenedioxyamphetamine/*pharmacology
MH  - Paroxetine/pharmacology
MH  - Phenols/pharmacology
MH  - RNA, Messenger/*biosynthesis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Dopamine D1/antagonists & inhibitors
MH  - Receptors, N-Methyl-D-Aspartate/antagonists & inhibitors
MH  - Serotonin Antagonists/pharmacology
MH  - Selective Serotonin Reuptake Inhibitors/pharmacology
MH  - Transcription Factors/*biosynthesis
EDAT- 2000/08/26 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/08/26 11:00
PHST- 2000/08/26 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/08/26 11:00 [entrez]
AID - S0014299900005215 [pii]
AID - 10.1016/s0014-2999(00)00521-5 [doi]
PST - ppublish
SO  - Eur J Pharmacol. 2000 Aug 25;402(3):215-22. doi: 10.1016/s0014-2999(00)00521-5.