PMID- 10960730
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240109
IS  - 1873-2259 (Electronic)
IS  - 0168-9452 (Linking)
VI  - 157
IP  - 2
DP  - 2000 Aug 22
TI  - Nitric oxide induces cell death in Taxus cells.
PG  - 173-180
AB  - Sodium nitroprusside (SNP), a nitric oxide donor, or centrifugation at 150 times 
      unit gravity, caused a nitric oxide burst in oocyte-derived Taxus brevifolia 
      haploid cultures. This burst, visualized by the specific fluorescent probe 
      4,5-diaminofluorescein diacetate (DAF-2 DA), preceded a significant increase in 
      nuclear DNA fragmentation and cell death. DNA fragmentation was detected in situ 
      by the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling 
      (TUNEL) of DNA 3'-OH groups. Nitric oxide formation and cell death were 
      significantly decreased by N(G)-monomethyl-L-arginine (L-NMMA), a nitric 
      oxide-synthase (NOS; EC 1.14.13.39) inhibitor. Our results show that nitric oxide 
      leads to irreversible DNA fragmentation and cell death under stressful 
      conditions, and that its effect can be prevented by L-NMMA.
FAU - Pedroso, MC
AU  - Pedroso MC
AD  - Department Biologia Vegetal, Centro de Biotecnologia Vegetal, Universidade de 
      Lisboa, Ed. C2, Piso 1, Campo Grande, P-1749-016, Lisbon, Portugal
FAU - Magalhaes, JR
AU  - Magalhaes JR
FAU - Durzan, D
AU  - Durzan D
LA  - eng
PT  - Journal Article
PL  - Ireland
TA  - Plant Sci
JT  - Plant science : an international journal of experimental plant biology
JID - 9882015
EDAT- 2000/08/29 00:00
MHDA- 2000/08/29 00:01
CRDT- 2000/08/29 00:00
PHST- 2000/08/29 00:00 [pubmed]
PHST- 2000/08/29 00:01 [medline]
PHST- 2000/08/29 00:00 [entrez]
AID - S0168945200002788 [pii]
AID - 10.1016/s0168-9452(00)00278-8 [doi]
PST - ppublish
SO  - Plant Sci. 2000 Aug 22;157(2):173-180. doi: 10.1016/s0168-9452(00)00278-8.