PMID- 10960766 OWN - NLM STAT- MEDLINE DCOM- 20001031 LR - 20190720 IS - 0304-3835 (Print) IS - 0304-3835 (Linking) VI - 158 IP - 2 DP - 2000 Oct 1 TI - An improved rapid procedure for fluorescence in situ hybridization that is applicable to intraoperative cancer cytodiagnosis. PG - 165-9 AB - Fluorescence in situ hybridization (FISH) is among the most simple and useful methods for detecting numerical and structural aberration of chromosomes but it requires 12-24 h to complete. We devised a rapid FISH method that can be performed within 2 h. Here we describe the technique, which we have found to be extreme simple and as sensitive and specific as standard FISH, making it highly suitable for clinical use. FAU - Koide, K AU - Koide K AD - Department of Digestive Surgery, Kyoto Prefectural University of Medicine, 465 Kajii-cho, Hirokohji, Kawaramachi-tori, Kamigyo-ku, Kyoto, Japan. koide@iwa.att.ne.jp FAU - Sakakura, C AU - Sakakura C FAU - Hagiwara, A AU - Hagiwara A FAU - Yamaguchi, T AU - Yamaguchi T FAU - Yamagishi, H AU - Yamagishi H FAU - Abe, T AU - Abe T FAU - Inazawa, J AU - Inazawa J LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Ireland TA - Cancer Lett JT - Cancer letters JID - 7600053 SB - IM MH - Adult MH - Aged MH - Aged, 80 and over MH - Allelic Imbalance MH - Chromosome Aberrations MH - Chromosomes, Human, Pair 1/genetics MH - Chromosomes, Human, Pair 11/genetics MH - Chromosomes, Human, Pair 17/genetics MH - Chromosomes, Human, Pair 18/genetics MH - Colorectal Neoplasms/genetics/pathology MH - *Cytodiagnosis MH - Diagnosis, Differential MH - Esophageal Neoplasms/genetics/pathology MH - Female MH - Humans MH - In Situ Hybridization, Fluorescence/*methods MH - Male MH - Middle Aged MH - Neoplasms/genetics/*pathology MH - Sensitivity and Specificity MH - Stomach Neoplasms/genetics/pathology MH - Time Factors MH - U937 Cells MH - Y Chromosome/genetics EDAT- 2000/08/29 11:00 MHDA- 2001/02/28 10:01 CRDT- 2000/08/29 11:00 PHST- 2000/08/29 11:00 [pubmed] PHST- 2001/02/28 10:01 [medline] PHST- 2000/08/29 11:00 [entrez] AID - S0304383500005164 [pii] AID - 10.1016/s0304-3835(00)00516-4 [doi] PST - ppublish SO - Cancer Lett. 2000 Oct 1;158(2):165-9. doi: 10.1016/s0304-3835(00)00516-4.