PMID- 10961660
OWN - NLM
STAT- MEDLINE
DCOM- 20010531
LR  - 20190701
IS  - 0304-3940 (Print)
IS  - 0304-3940 (Linking)
VI  - 289
IP  - 3
DP  - 2000 Aug 11
TI  - Decreased cortical levels of astrocytic glutamate transport protein GLT-1 in a 
      rat model of posttraumatic epilepsy.
PG  - 185-8
AB  - The extracellular homeostasis of glutamate in the brain is maintained by the 
      efficient uptake into astroglial cells. The high extracellular glutamate levels 
      seen during seizures are therefore probably a result of both an increased 
      synaptic release and a deranged glutamate uptake. In this study we used 
      immuno-blotting technique to measure the cortical levels of the astrocytic 
      glutamate transport protein (GLT-1) and of the glutamate and aspartate 
      transporting protein (GLAST) in an epilepsy model induced by ferrous chloride 
      injection in the cortex of rats. The levels of GLT-1 were lower in epileptic rats 
      than in controls, day 1 and 5 after induction, but not at 3 months. Glial 
      fibrillary protein (GFAP) levels increased with time in the epileptic model, 
      whereas GLAST and beta-tubulin III remained unchanged compared to controls. The 
      results suggest that the transient decrease of GLT-1 could play a role in 
      epileptogenesis, while recurrent seizure activity may be maintained by other 
      mechanisms.
FAU - Samuelsson, C
AU  - Samuelsson C
AD  - Department of Neuroscience, Uppsala University, Sweden.
FAU - Kumlien, E
AU  - Kumlien E
FAU - Flink, R
AU  - Flink R
FAU - Lindholm, D
AU  - Lindholm D
FAU - Ronne-Engstrom, E
AU  - Ronne-Engstrom E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (Amino Acid Transport System X-AG)
RN  - 0 (Ferrous Compounds)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (Tubulin)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - S3Y25PHP1W (ferrous chloride)
SB  - IM
MH  - ATP-Binding Cassette Transporters/*metabolism
MH  - Amino Acid Transport System X-AG
MH  - Animals
MH  - Astrocytes/drug effects/*metabolism/pathology
MH  - Cerebral Cortex/*metabolism/pathology/physiopathology
MH  - Disease Models, Animal
MH  - Electroencephalography/drug effects
MH  - Epilepsy, Post-Traumatic/chemically induced/*metabolism/physiopathology
MH  - Ferrous Compounds/adverse effects
MH  - Glial Fibrillary Acidic Protein/metabolism
MH  - Glutamic Acid/*metabolism
MH  - Male
MH  - Neurons/drug effects/metabolism/pathology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Tubulin/metabolism
EDAT- 2000/08/29 11:00
MHDA- 2001/06/02 10:01
CRDT- 2000/08/29 11:00
PHST- 2000/08/29 11:00 [pubmed]
PHST- 2001/06/02 10:01 [medline]
PHST- 2000/08/29 11:00 [entrez]
AID - S0304394000012842 [pii]
AID - 10.1016/s0304-3940(00)01284-2 [doi]
PST - ppublish
SO  - Neurosci Lett. 2000 Aug 11;289(3):185-8. doi: 10.1016/s0304-3940(00)01284-2.