PMID- 10963352
OWN - NLM
STAT- MEDLINE
DCOM- 20000914
LR  - 20200324
IS  - 0304-8608 (Print)
IS  - 1432-8798 (Electronic)
IS  - 0304-8608 (Linking)
VI  - 145
IP  - 7
DP  - 2000
TI  - Restriction fragment length polymorphism analysis of open reading frame 5 gene of 
      porcine reproductive and respiratory syndrome virus isolates in Korea.
PG  - 1481-8
AB  - The genetic variability of porcine reproductive and respiratory syndrome virus 
      (PRRSV) was studied by restriction fragment length polymorphism (RFLP) of 
      polymerase chain reaction (PCR)-amplified fragments among 50 Korean isolates from 
      open reading frame 5. All Korean PRRSVs were isolated from the field cases after 
      the marketing of an U.S. ATCC VR2332-derived modified live PRRSV vaccine. 
      Combining the restriction enzyme digestion patterns obtained with MluI, HincII, 
      SacII, and HaeIII, we observed 19 distinct RFLP patterns. Seventeen out of 50 
      PRRSV isolates (34%) exhibited the modified live PRRSV vaccine RFLP pattern. The 
      genomic variations that have been identified in the present study seemed to 
      represent characteristic features of the Korean PRRSV isolates. PCR-based RFLP 
      analysis using several restriction enzymes provides a good genetic estimate for 
      isolate differentiation.
FAU - Cheon, D S
AU  - Cheon DS
AD  - Department of Veterinary Pathology, College of Veterinary Medicine and School of 
      Agricultural Biotechnology, Seoul National University, Suwon, Republic of Korea.
FAU - Chae, C
AU  - Chae C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
SB  - IM
MH  - Animals
MH  - *Genes, Viral
MH  - Genome, Viral
MH  - Korea/epidemiology
MH  - Open Reading Frames/genetics
MH  - Polymorphism, Restriction Fragment Length
MH  - Porcine Reproductive and Respiratory Syndrome/epidemiology/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/isolation & 
      purification
MH  - Swine
MH  - Swine Diseases/epidemiology
PMC - PMC7087178
EDAT- 2000/08/30 11:00
MHDA- 2000/09/19 11:01
PMCR- 2020/03/23
CRDT- 2000/08/30 11:00
PHST- 2000/08/30 11:00 [pubmed]
PHST- 2000/09/19 11:01 [medline]
PHST- 2000/08/30 11:00 [entrez]
PHST- 2020/03/23 00:00 [pmc-release]
AID - 01451481.705 [pii]
AID - 10.1007/s007050070105 [doi]
PST - ppublish
SO  - Arch Virol. 2000;145(7):1481-8. doi: 10.1007/s007050070105.