PMID- 10963894 OWN - NLM STAT- MEDLINE DCOM- 20001004 LR - 20190701 IS - 0304-3940 (Print) IS - 0304-3940 (Linking) VI - 290 IP - 3 DP - 2000 Sep 1 TI - The endogenous survival promotion of axotomized rat corticospinal neurons by brain-derived neurotrophic factor is mediated via paracrine, rather than autocrine mechanisms. PG - 185-8 AB - The previously reported rescue of corticospinal neurons (CSN) from axotomy-induced death by intracortical glial cell line-derived neurotrophic factor (GDNF)- and neurotrophin-3 (NT-3)-infusions depends on endogenous cortical brain-derived neurotrophic factor (BDNF). The present study examines whether BDNF, GDNF, or NT-3 can stimulate an autocrine or paracrine BDNF-support of lesioned CSN. BDNF-infusions increase BDNF mRNA-expression throughout cortical layers 2-5 and NT-3-treatment results in upregulation of BDNF-transcripts in the upper cortical layers. In contrast, GDNF-treatment had no effect. While virtually all CSN express the BDNF-receptor trkB, less than half of them express BDNF, and these expression patterns are unchanged after axotomy and the different neurotrophic factor treatments. The findings suggest that axotomized CSN are supported via a paracrine BDNF-mechanism which can be stimulated by BDNF- and NT-3-, but not by GDNF. FAU - Schutte, A AU - Schutte A AD - Department of Anatomy, University of Saarland, D-66421 Homburg/, Saar, Germany. FAU - Yan, Q AU - Yan Q FAU - Mestres, P AU - Mestres P FAU - Giehl, K M AU - Giehl KM LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Ireland TA - Neurosci Lett JT - Neuroscience letters JID - 7600130 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Gdnf protein, rat) RN - 0 (Glial Cell Line-Derived Neurotrophic Factor) RN - 0 (Nerve Growth Factors) RN - 0 (Nerve Tissue Proteins) RN - 0 (Neurotrophin 3) SB - IM MH - Animals MH - Autocrine Communication/physiology MH - Axotomy/*adverse effects MH - Brain-Derived Neurotrophic Factor/*metabolism/pharmacology MH - Cell Survival/drug effects/*physiology MH - Cerebral Cortex/drug effects/pathology/physiopathology MH - Denervation MH - Glial Cell Line-Derived Neurotrophic Factor MH - Male MH - *Nerve Growth Factors MH - Nerve Tissue Proteins/metabolism/pharmacology MH - Neurons/drug effects/*metabolism/pathology MH - Neurotrophin 3/metabolism/pharmacology MH - Paracrine Communication/*physiology MH - Pyramidal Tracts/drug effects/injuries/pathology/*physiopathology MH - Rats MH - Rats, Sprague-Dawley MH - Regeneration/drug effects/*physiology EDAT- 2000/08/30 11:00 MHDA- 2000/10/07 11:01 CRDT- 2000/08/30 11:00 PHST- 2000/08/30 11:00 [pubmed] PHST- 2000/10/07 11:01 [medline] PHST- 2000/08/30 11:00 [entrez] AID - S0304394000013513 [pii] AID - 10.1016/s0304-3940(00)01351-3 [doi] PST - ppublish SO - Neurosci Lett. 2000 Sep 1;290(3):185-8. doi: 10.1016/s0304-3940(00)01351-3.