PMID- 10964327
OWN - NLM
STAT- MEDLINE
DCOM- 20000919
LR  - 20190620
IS  - 0008-543X (Print)
IS  - 0008-543X (Linking)
VI  - 89
IP  - 5
DP  - 2000 Sep 1
TI  - Expression of transforming growth factor-alpha mRNA in livers of patients with 
      chronic viral hepatitis and hepatocellular carcinoma.
PG  - 977-82
AB  - BACKGROUND: Transforming growth factor-alpha (TGFalpha) is an important autocrine 
      growth factor of hepatocytes. The authors evaluated the roles of TGFalpha in 
      chronic viral hepatitis (CVH) and hepatocellular carcinoma (HCC). METHODS: The 
      authors measured the amounts of TGFalpha mRNA in liver tissues from 18 patients 
      with HCC, 31 patients with CVH, and 7 normal controls. " Hot-start" reverse 
      transcription-polymerase chain reaction (RT-PCR) using oligo-dT and specific 
      primers detected TGFalpha mRNA in total cellular RNA extracted from liver 
      tissues. The levels of TGFalpha mRNA were determined by the end point titers of 
      serial, two-fold dilutions of cDNA. The amounts of hepatitis B virus RNA 
      (HBV-RNA) in livers of patients with chronic hepatitis B also were measured by 
      Northern blot hybridization. RESULTS: TGFalpha mRNA levels were extremely higher 
      in patients with HCC compared with patients with CVH and normal controls, and the 
      levels in patients with CVH also were elevated compared with normal controls. The 
      levels of TGFalpha mRNA were overexpressed in the underlying livers of patients 
      with HCC compared with patients with CVH, although they were lower than those 
      found in HCC tissues. The levels of TGFalpha mRNA were higher in samples from 
      patients with chronic hepatitis B than in samples from patients with chronic 
      hepatitis C. The levels of TGFalpha mRNA were not correlated with serum alanine 
      aminotransferase or HBV-RNA levels in liver tissues in patients with chronic 
      hepatitis B. However, the expression of TGFalpha mRNA tended to be higher in the 
      livers of patients with raised serum alpha-fetoprotein levels. CONCLUSIONS: The 
      overexpression of TGFalpha mRNA in the liver seems to be associated with the 
      regeneration of hepatocytes rather than hepatic necrosis or viral replication. 
      Also, it may be related closely to the development or progression of HCC, 
      especially in the livers of patients with chronic hepatitis B.
FAU - Chung, Y H
AU  - Chung YH
AD  - Department of Internal Medicine, University of Ulsan College of Medicine, Seoul, 
      Korea.
FAU - Kim, J A
AU  - Kim JA
FAU - Song, B C
AU  - Song BC
FAU - Lee, G C
AU  - Lee GC
FAU - Koh, M S
AU  - Koh MS
FAU - Lee, Y S
AU  - Lee YS
FAU - Lee, S G
AU  - Lee SG
FAU - Suh, D J
AU  - Suh DJ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer
JT  - Cancer
JID - 0374236
RN  - 0 (RNA, Messenger)
RN  - 0 (Transforming Growth Factor alpha)
SB  - IM
MH  - Adult
MH  - Carcinoma, Hepatocellular/complications/*metabolism/virology
MH  - Chronic Disease
MH  - Disease Progression
MH  - Female
MH  - Hepatitis, Viral, Human/complications/*metabolism
MH  - Humans
MH  - Liver/metabolism
MH  - Liver Neoplasms/complications/*metabolism/virology
MH  - Male
MH  - Middle Aged
MH  - RNA, Messenger/biosynthesis
MH  - Statistics as Topic
MH  - Transforming Growth Factor alpha/*biosynthesis/genetics
EDAT- 2000/08/30 11:00
MHDA- 2000/09/23 11:01
CRDT- 2000/08/30 11:00
PHST- 2000/08/30 11:00 [pubmed]
PHST- 2000/09/23 11:01 [medline]
PHST- 2000/08/30 11:00 [entrez]
AID - 10.1002/1097-0142(20000901)89:5<977::AID-CNCR6>3.0.CO;2-I [pii]
AID - 10.1002/1097-0142(20000901)89:5<977::aid-cncr6>3.0.co;2-i [doi]
PST - ppublish
SO  - Cancer. 2000 Sep 1;89(5):977-82. doi: 
      10.1002/1097-0142(20000901)89:5<977::aid-cncr6>3.0.co;2-i.