PMID- 10964714
OWN - NLM
STAT- MEDLINE
DCOM- 20001003
LR  - 20081121
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 275
IP  - 2
DP  - 2000 Aug 28
TI  - Genomic structure and in vivo expression of the human organic anion transporter 1 
      (hOAT1) gene.
PG  - 623-30
AB  - The human organic anion transporter 1 (hOAT1) plays a key role in the secretion 
      of an array of potentially toxic organic anions including many clinically 
      important drugs. Here we report on the genomic cloning of hOAT1. A human genomic 
      library was used for screening of a PAC (P1 artificial chromosome) clone applying 
      PCR techniques. Sequencing of several restriction subclones and of a 
      PCR-generated clone revealed that the hOAT1 gene spans 8.2 kb and is composed of 
      10 exons divided by 9 introns. RT-PCR studies in a human kidney specimen led to 
      the detection of two new splice variants, hOAT1-3 and hOAT1-4, showing a 132-bp 
      in-frame deletion. Using fluorescence in situ hybridization (FISH) we mapped the 
      hOAT1 gene as a single signal to chromosome 11q13.1-q13.2. Additionally, 600 bp 
      of the 5' flanking region was analyzed, illustrating the probable transcription 
      start site at nt -280, a NF-kappaB-site at nt -397 and several putative 
      transcription factor binding sites.
CI  - Copyright 2000 Academic Press.
FAU - Bahn, A
AU  - Bahn A
AD  - Zentrum fur Physiologie und Pathophysiologie, Humboldtallee 23, Gottingen, 37073, 
      Germany. abahn@veg-physiol.med.uni-goettingen.de
FAU - Prawitt, D
AU  - Prawitt D
FAU - Buttler, D
AU  - Buttler D
FAU - Reid, G
AU  - Reid G
FAU - Enklaar, T
AU  - Enklaar T
FAU - Wolff, N A
AU  - Wolff NA
FAU - Ebbinghaus, C
AU  - Ebbinghaus C
FAU - Hillemann, A
AU  - Hillemann A
FAU - Schulten, H J
AU  - Schulten HJ
FAU - Gunawan, B
AU  - Gunawan B
FAU - Fuzesi, L
AU  - Fuzesi L
FAU - Zabel, B
AU  - Zabel B
FAU - Burckhardt, G
AU  - Burckhardt G
LA  - eng
SI  - GENBANK/AJ249369
SI  - GENBANK/AJ251529
SI  - GENBANK/AJ271205
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Anion Transport Proteins)
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA Primers)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Anion Transport Proteins
MH  - Base Sequence
MH  - Carrier Proteins/*genetics
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 11
MH  - DNA
MH  - DNA Primers
MH  - Exons
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Introns
MH  - Molecular Sequence Data
MH  - Promoter Regions, Genetic
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2000/08/31 11:00
MHDA- 2000/10/07 11:01
CRDT- 2000/08/31 11:00
PHST- 2000/08/31 11:00 [pubmed]
PHST- 2000/10/07 11:01 [medline]
PHST- 2000/08/31 11:00 [entrez]
AID - S0006-291X(00)93230-3 [pii]
AID - 10.1006/bbrc.2000.3230 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2000 Aug 28;275(2):623-30. doi: 
      10.1006/bbrc.2000.3230.